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177,170 results

Showing: 10701 - 10750 of 177170 results

  1. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    123366-plasmid-map-sequence-id-242548

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  4. mIL12-N1

    Plasmid
    #123139
    Purpose
    Mammalian expression of murine cytokine IL-12 under CMV promoter
    Depositor
    Jordan Green
    Publication
    Tzeng et al Proc Natl Acad Sci U S A. 2020 Feb 7. pi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IL12 tandem p40 p35
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    123139-plasmid-map-sequence-id-238577

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  11. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  15. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112139-plasmid-map-sequence-id-217823

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  18. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    102886-plasmid-map-sequence-id-194712

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  23. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Publication
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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  32. pCMV-BE-zCas9

    Plasmid
    #101739
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    101739-plasmid-map-sequence-id-198316

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  37. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  40. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92065-plasmid-map-sequence-id-181210

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  41. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Publication
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  44. pSG5-HA-p300-DY1399

    Plasmid
    #89095
    Purpose
    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect
    Depositor
    Elizabeth Wilson
    Publication
    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300-D1399Y mutant (EP300 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    p300-DY1399
    Promoter
    SV40
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89095-plasmid-map-sequence-id-373226

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Showing: 10701 - 10750 of 177170 results