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171,035 results

Showing: 10701 - 10750 of 171035 results

  5. pGCNK58

    Plasmid
    #202461
    Purpose
    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
    Depositor
    Stephen Harrison, Arthur Palmer
    Article
    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi
    Insert
    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Dec. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202461-plasmid-map-sequence-id-403118

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  9. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Available Since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

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  25. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid
    #174659
    Purpose
    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    Xon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174659-plasmid-map-sequence-id-351621

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  28. CNR2-Tango

    Plasmid
    #66255
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CNR2 (CNR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. BRD3

    Plasmid
    #38941
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD3 (BRD3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid
    #59356
    Purpose
    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59356-plasmid-map-sequence-id-94885

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  37. GuideVector_BbsI

    Plasmid
    #110692
    Purpose
    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.
    Depositor
    Douglas Mahoney
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110692-plasmid-map-sequence-id-211319

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  39. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  47. pGAPTrap-eGFP-IRESMuro

    Plasmid
    #82504
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMuro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82504-plasmid-map-sequence-id-156573

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  50. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available Since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    40025-plasmid-map-sequence-id-210933

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Showing: 10701 - 10750 of 171035 results