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pCAG eGFP-Oct6

Plasmid

#203790

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou3f1 (Pou3f1 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-M2rtTA-T2A-Puro

Plasmid

#169175

Purpose

piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

Depositor

Igor Slukvin

Article

Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

Insert

M2rtTA-T2A-Puro

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

Plasmid

#124870

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV dCas9-MC (eGFP)

Plasmid

#89931

Purpose

Expresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC

Use

Tags

Flag

Expression

Mammalian

Mutation

deactivated Cas9, fragment of M.SssI (residues 27…

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB4-3

Plasmid

#121536

Purpose

sgITGB4-3 sequence: GAGGAGCGTAGGTCCTCGCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB4-3

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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DTECT - Plasmid for Standard Curve

Plasmid

#139333

Purpose

This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

DTECT ligation product

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp92

Plasmid

#157975

Purpose

pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

Plasmid

#105025

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

Plasmid

#105026

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C192A-Myc

Plasmid

#201168

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67290

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PKA-RIIβ-sREACh

Plasmid

#114113

Purpose

Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

Plasmid

#188391

Purpose

Reporter for BCAM expression

Depositor

Markus Frank

Article

Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

Insert

BCAM reporter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-TAZ CA

Plasmid

#79504

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WWTR1 (WWTR1 Human)

Use

Multisite gateway entry clone

Tags

3xFLAG

Expression

Mutation

S89A

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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