19,773 results

Showing: 6451 - 6480 of 19773 results

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pLentiPGK Puro DEST p38KTRClover

Plasmid

#59152

Purpose

Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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SV-ABAactDA

Plasmid

#38247

Depositor

Jerry Crabtree

Article

Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

Inserts

VP16/PYL1
Gal4DBD/ABI*

Tags

Flag, Gal4DBD, HA, and VP16

Expression

Mammalian

Mutation

D143A

Promoter

SV40 (IRES) and SV40p

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima-P2A-FRB-Fis1

Plasmid

#135295

Purpose

stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-TOMM20-N-10

Plasmid

#123284

Purpose

Localization: Outer Mitochondrial Membrane

Depositor

Kevin McGowan

Insert

TOMM20 (TOMM20 Human)

Tags

HaloTag7

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-C1

Plasmid

#54768

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTC-ApoE-Tet

Plasmid

#85578

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Insert

CreER

Use

Cre/Lox

Expression

Mammalian

Promoter

ApoE.HCR.hAAT

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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GABA(A) receptor subunit a1SE

Plasmid

#49168

Purpose

pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

Insert

GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)

Tags

myc and pHGFP (pHIuorin)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-CoV-2-S-RBD-8his

Plasmid

#145145

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Tags

8his tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Green Lindoblum

Plasmid

#163113

Purpose

Expresses biosensor for lactate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Lindolum

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EM2K

Plasmid

#20923

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-N 16E6

Plasmid

#37875

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-puro-CMV-GC3AI

Plasmid

#78910

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

GC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLZF

Plasmid

#61543

Purpose

Expresses PLZF in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLZF (ZBTB16 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

I380T and G580D

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA1-CH-mneo

Plasmid

#51288

Purpose

contains the fully codon optimized ORF1 and ORF2 of the human L1RP

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 (L1RE1 Human)

Tags

mneoI indicator cassette containing an inverted n…

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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ClpP-his

Plasmid

#22144

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

ClpP-His6

Tags

his

Expression

Bacterial

Mutation

C-terminal His tag

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP N10 TEV LIC cloning vector (2C-T)

Plasmid

#29706

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA2.0

Plasmid

#158394

Purpose

Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCWXPG-UBI-Ngn3PDX1MafA

Plasmid

#114303

Purpose

Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

Ngn3 (NEUROG3 Human)

Use

Lentiviral

Mutation

Ngn3, PDX1 and MafA proteins are expressed from a…

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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tol2-mpeg1-mcherry

Plasmid

#58935

Purpose

labels macrophages with mcherry

Depositor

Anna Huttenlocher

Insert

mpeg1-mcherry

Promoter

mpeg1

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6 (fl)

Plasmid

#137816

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

R296K in the V6 region [Please see depositor comm…

Promoter

PGK

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

Plasmid

#162381

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CAG

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-RNF2

Plasmid

#126544

Purpose

Expresses GFP-tagged RNF2 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RING1B (RNF2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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19,773 results