We narrowed to 22,191 results for: his

1. pET11a-STEPtag

   Plasmid

   #159373

   Purpose

   Expresses STEPtag in E. coli

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK_proB_eGFP

   Plasmid

   #59845

   Purpose

   Constitutively expresses eGFP with proB promoter

   Depositor

   Timothy Whitehead

   Article

   Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   S2R,S65G,S72A

   Promoter

   proB

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21d-colEwt2/Imwt2

   Plasmid

   #107127

   Purpose

   plasmid for expression of WT colicin endonuclease and immunity pair 2

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEwt2/Imwt2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP S2TAG/E17TAG

   Plasmid

   #68298

   Purpose

   S2TAG/E17TAG GFP reporter

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   GFP S2TAG/E17TAG
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-22b(+)-UTPase

   Plasmid

   #12667

   Depositor

   SeWon Suh

   Article

   Han et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   dUTP pyrophosphatase (DUT1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET30b-7D-SrtA

   Plasmid

   #105600

   Purpose

   expresses 7D sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7D mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK_proK1_eGFP

   Plasmid

   #59853

   Purpose

   Constitutively expresses eGFP with proK1 promoter

   Depositor

   Timothy Whitehead

   Article

   Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   S2R,S65G,S72A

   Promoter

   proK1

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Y-RAN-RFP6

   Plasmid

   #106424

   Purpose

   Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

   Insert

   Y-RAN-RFP6
   

   Use

   Tags

   HA and His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tIVR

   Plasmid

   #122663

   Purpose

   Express tIVR protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tIVR
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. GFP Q157TAG

    Plasmid

    #68297

    Purpose

    Q157TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    GFP Q157TAG
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-LSSA12

    Plasmid

    #186546

    Purpose

    LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSA12
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-G65S/Y203I/Q204E/E222D/R223F

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSKB2-TRex

    Plasmid

    #31349

    Depositor

    Clara Kielkopf

    Article

    McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

    Insert

    Thermus aquaticus Redox Sensing Repressor
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 RAD54B

    Plasmid

    #67124

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAD54B (RAD54B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLIA-E p27 (CC#620)

    Plasmid

    #15191

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-H-RAN-GFP1

    Plasmid

    #106418

    Purpose

    H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    H-RAN-GFP1
    

    Use

    Tags

    HA, His, and Multiple HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYES2.1-cerMET6

    Plasmid

    #107438

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET6 (MET6 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYES2.1-pomSua1

    Plasmid

    #107446

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Sua1
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    See depositor comments below.

    Promoter

    GAL1

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYES2.1-pomMet14

    Plasmid

    #107451

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met14 (met14 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYES2.1-pomMet1

    Plasmid

    #107444

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met1
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST26-hOPTN-D474N

    Plasmid

    #23051

    Depositor

    Jon Ashwell

    Article

    Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

    Insert

    Optineurin (OPTN Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pMVV094

    Plasmid

    #215302

    Purpose

    Expresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSETA_crE6G2

    Plasmid

    #172929

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6G2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Val317Gly, Met316Gly

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFBXC3GH

    Plasmid

    #49051

    Purpose

    FX cloning insect cell expression vector

    Depositor

    Markus Gruetter

    Article

    Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Insect

    Mutation

    Promoter

    polH (polyhedrin)

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB401NL3F10H

    Plasmid

    #141285

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSYN-Kex-002

    Plasmid

    #42504

    Depositor

    Robert Peters

    Article

    Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

    Insert

    Kex2 protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pYES2.1-cerMET16

    Plasmid

    #107442

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET16 (MET16 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Ac5-STABLE1-puro

    Plasmid

    #208031

    Purpose

    Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBbA5k-EPL123

    Plasmid

    #45361

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    AcrAB
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available Since

    June 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC-Per

    Plasmid

    #33157

    Depositor

    Michael Rosbash

    Article

    Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

    Insert

    Per (per Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pCMV-Y-RAN-GFP1

    Plasmid

    #106422

    Purpose

    Y-RAN-GFP1 (RANbody GFP1::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-GFP1
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYES2.1-cerMET10

    Plasmid

    #107440

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET10 (MET10 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEcCas6

    Plasmid

    #170091

    Purpose

    expresses E. coli type I-E Cas6

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cas6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-gLuc-C1C2

    Plasmid

    #178426

    Purpose

    Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    gLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNM3689

    Plasmid

    #154111

    Purpose

    miniMos single component RMCE landing site vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
    

    Use

    Minimos

    Tags

    Expression

    Mutation

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28b-pvdA WT

    Plasmid

    #87098

    Purpose

    expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

    Insert

    pvdA WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBAD-RDSmCherry1

    Plasmid

    #89987

    Purpose

    Red-Shifted variants of red fluorescent protein mCherry

    Depositor

    Robert Campbell

    Article

    Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

    Insert

    RDSmCherry1
    

    Use

    Tags

    6xHis-T7-Xpress

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge