We narrowed to 5,494 results for: SPI

1. 6His-MBP-TEV-huLbCpf1

   Plasmid

   #90096

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Insert

   huLbCpf1
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SARS-CoV-2 5'UTR-Luciferase

   Plasmid

   #191480

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   contains SARS-CoV-2 5' UTR

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mEGFP-CaMKIIa (T286D)

   Plasmid

   #127391

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV-hNG2-GFP

   Plasmid

   #183910

   Purpose

   Expression of GFP under the human NG2 promoter

   Depositor

   Chun-Li Zhang

   Article

   Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

   Insert

   GFP (GFAP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mEGFP-CaMKIIa (T286A)

   Plasmid

   #127390

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBEI-6409

   Plasmid

   #47048

   Purpose

   BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacUV5

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-Neo-ISG15-LRAA

   Plasmid

   #80405

   Purpose

   overexpression of nonconjugative ISG15

   Depositor

   Isidoro Martinez

   Article

   Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nonconjugative ISG15, LRAA

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PR_GEF (2XPDZ-mCherry-Larg (DH)

    Plasmid

    #80407

    Purpose

    Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    DH domain only (aa 766-997)

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-ch-TOG-His6

    Plasmid

    #69108

    Purpose

    For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP

    Plasmid

    #92388

    Purpose

    Cell death reporter

    Depositor

    Nicholas Cole

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    ANNEXINV (ANXA5 Human)
    

    Use

    Zebrafish expression

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    ubb

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-J chain

    Plasmid

    #145146

    Purpose

    Mammalian expression plasmid for J chain of polymeric antibody classes

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    immunoglobulin J chain (JCHAIN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCF160_VSV-G

    Plasmid

    #225963

    Purpose

    CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-VSVG (env protein)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRES2Cx32WTEGFP

    Plasmid

    #78150

    Purpose

    Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

    Depositor

    Mario Bortolozzi

    Article

    Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

    Insert

    Connexin 32 (GJB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJBEI-6410

    Plasmid

    #47049

    Purpose

    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. aMHC-mCherry-Rex-Blasticidin

    Plasmid

    #21228

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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