We narrowed to 43,251 results for: cha;

1. pXylA-agrCA-IV

   Plasmid

   #53438

   Purpose

   xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-parkin F463A

   Plasmid

   #45975

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin F463A (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   tac

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-miSFIT-2x

   Plasmid

   #124691

   Purpose

   Tuning expression of EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pr2-T7RNAP

   Plasmid

   #67739

   Purpose

   OR2-OR1-P2 promoter expressing T7 RNAP

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-T7RNAP-T500
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr2

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTXG-hTDG-K330A

   Plasmid

   #52282

   Purpose

   Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   hTDG (TDG Human)
   

   Use

   Tags

   GyrA-intein-GST

   Expression

   Bacterial

   Mutation

   K330A, SUMO acceptor site mutation

   Promoter

   T7

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMpGWB330

   Plasmid

   #68658

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   tdTomato

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL1-PorA-4xDQNAT

   Plasmid

   #128392

   Purpose

   In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   PorA
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-TTc-4xDQNAT

   Plasmid

   #128393

   Purpose

   In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin fragment C domain
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAP1_Ta_Yr7_CDS_no_STOP

   Plasmid

   #141093

   Purpose

   Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

   Depositor

   Cristobal Uauy

   Article

   Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

   Insert

   Ta_Yr7
   

   Use

   Unspecified; Level 0 module for golden gate cloni…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TU#1450_unc-53a_cDNA

    Plasmid

    #82361

    Purpose

    express unc-53a cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-53 a isoform (unc-53 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pF(UG) hSyn SYP-SELF

    Plasmid

    #158770

    Purpose

    Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptophysin 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMpGWB223

    Plasmid

    #68614

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Triple repeats of Citrine (3xCitrine)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGWB226

    Plasmid

    #68617

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV5 KSR1 T274V

    Plasmid

    #25975

    Depositor

    Rob Lewis

    Article

    Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonine 274 to valine

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pMpGWB209

    Plasmid

    #68600

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-Hemmar-FLP(D)

    Plasmid

    #119895

    Purpose

    FLP(D) with a hsp70 promoter

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Insert

    FLP(D)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    mutated form of FLP, which at position 5 contains…

    Promoter

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB429

    Plasmid

    #68694

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFSW Syt1-C2A IRES GFP

    Plasmid

    #133818

    Purpose

    Encodes the C2A domain of synaptotagmin 1 for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMDC84-SEIPIN1

    Plasmid

    #96979

    Purpose

    Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHU6_Ch13_102010574-gRNA-rev

    Plasmid

    #81219

    Purpose

    pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporter

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3186 pBS Bcl-XL Exon 1

    Plasmid

    #8755

    Depositor

    Stanley Korsmeyer

    Article

    Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

    Insert

    Bcl-XL (Exon 1) (Bcl2l1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

24. pES1002

    Plasmid

    #21789

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Fus3-as2 (FUS3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 5' 1kb upstream UTR/promoter Q93…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)

    Plasmid

    #119064

    Purpose

    Cre/lox flip-out construct for marking cell clones in Tribolium castaneum

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    pUb regulatory sequence
    loxN
    mYFP
    loxN
    H2B-mcherry
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNMHCII-C2-GFP

    Plasmid

    #46043

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pHU6_gRNA_Ch10-rev2

    Plasmid

    #81207

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-hXRCC1-K176R

    Plasmid

    #52275

    Purpose

    Bacterial expression of human XRCC1-K176R with N-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    tac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCfB4613

    Plasmid

    #126752

    Purpose

    Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

    Depositor

    Michael Jensen

    Article

    Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

    Insert

    CDC14 (CDC14 Budding Yeast)
    

    Use

    Integrative plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTRX2_5xUAS

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSC-hTDG-K330A-PreE2

    Plasmid

    #52273

    Purpose

    Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Use

    Tags

    Ubc9-GST

    Expression

    Bacterial

    Mutation

    K330A, SUMO acceptor site mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Pr-delRBS-deGFP

    Plasmid

    #67744

    Purpose

    Pr promoter expressing a deGFP that is missing a ribosomal binding site

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    deGFP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTXG-hXRCC1-K176R

    Plasmid

    #52284

    Purpose

    Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GyrA-intein-GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX-parkin F146A

    Plasmid

    #45973

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin F146A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NHA-Delta 8

    Plasmid

    #36928

    Depositor

    Edward Chan

    Article

    Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

    Insert

    TNGW1 (TNRC6A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1195-1962 only

    Promoter

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

35. PGRP-LCx

    Plasmid

    #21058

    Depositor

    Johann Deisenhofer

    Article

    Chang et al Science. 2006 Mar 24. 311(5768):1761-4.

    Insert

    PGRP-LCx (PGRP-LC Fly)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA-N1-PDZ2-GAGA

    Plasmid

    #32857

    Depositor

    Edward Weinman

    Article

    Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

    Insert

    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
    

    Use

    Tags

    His,S-tag

    Expression

    Mammalian

    Mutation

    PDZ2 P(161)NGYGF mutated to H(161)NGAGA

    Promoter

    cmv

    Available since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHU6_Ch12_62418577-gRNA-rev

    Plasmid

    #81217

    Purpose

    pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCALNL-eGFP-Esp3I

    Plasmid

    #81226

    Purpose

    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NHA-Delta 11

    Plasmid

    #36926

    Depositor

    Edward Chan

    Article

    Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    aa 722-941 only

    Promoter

    Available since

    May 24, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pMpGWB412

    Plasmid

    #68677

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge