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Showing: 10801 - 10850 of 22610 results

  3. pCVD419

    Plasmid
    #63754
    Purpose
    3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.
    Depositor
    James Kaper
    Article
    Levine et al J Infect Dis. 1987 Jul;156(1):175-82.
    Insert
    ehxA
    Expression
    Bacterial
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pHL 1410

    Plasmid
    #53011
    Purpose
    pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    ompC
    rpoS
    Use
    Synthetic Biology
    Tags
    T7noRBS (900bp) and gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  8. pHL1853

    Plasmid
    #53040
    Purpose
    PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    LVA, RBS(st2), RBS(st3), and glgC leader
    Expression
    Bacterial
    Promoter
    pConNoHind and pLlac0-1
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pHL 990

    Plasmid
    #52992
    Purpose
    pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    RhyB sRNA (competitor)
    rpoS
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    includes −150 to +30 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pHL 1022

    Plasmid
    #52996
    Purpose
    pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    OxyS sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pHL 1020

    Plasmid
    #52995
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA (competitor)
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pHL 1019

    Plasmid
    #52994
    Purpose
    pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    MicC sRNA (Competitor)
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pHL 1039

    Plasmid
    #53000
    Purpose
    pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    OxyS sRNA (competitor)
    rpoS
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL 1073

    Plasmid
    #53003
    Purpose
    pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    ompC (target mRNA) and sodB (target mRNA)
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pHL 1303

    Plasmid
    #53008
    Purpose
    pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pHL1530

    Plasmid
    #37569
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    TetR
    CsrA
    Use
    Synthetic Biology
    Tags
    glgC leader region
    Expression
    Bacterial
    Mutation
    start codon removed
    Promoter
    pConNoHind and pLlacO-1
    Available Since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. pHL1355

    Plasmid
    #37566
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    TetR
    CsrA
    Use
    Synthetic Biology
    Tags
    glgC leader region
    Expression
    Bacterial
    Mutation
    start codon removed
    Promoter
    pConNoHind and pConNoHindM12
    Available Since
    July 26, 2012
    Availability
    Academic Institutions and Nonprofits only

  22. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  41. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    lacIq/Ptrc
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45944-plasmid-map-sequence-id-64657

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  42. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Promoter
    Trc (lactose/IPTG inducible)
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45940-plasmid-map-sequence-id-64653

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  47. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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Showing: 10801 - 10850 of 22610 results