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We narrowed to 52,925 results for: plasmid

Showing: 10801 - 10850 of 52925 results

  1. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

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  2. MSCV HA Akt loxP

    Plasmid
    #18767
    Depositor
    Scott Lowe
    Insert
    Akt (AKT1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only

  3. pAM-35s-CBL2-YFPc-1

    Plasmid
    #102394
    Purpose
    split YFP. Plant expression of CBL2-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CBL2 (CBL2 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102394-plasmid-map-sequence-id-196626

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  4. pLenti4/V5-DEST-P85(KS459del)

    Plasmid
    #40222
    Depositor
    Lynda Chin
    Insert
    P85(KS459delN) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    amino acids 459-460 replaced with asparagine
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only

  5. pT7-EGFP-C1-HseIF4E-F47AI79A_Z

    Plasmid
    #148136
    Purpose
    Mammalian Expression of HseIF4E-F47AI79A
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4E-F47AI79A (EIF4E Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148136-plasmid-map-sequence-id-390263

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  6. 4RG1

    Plasmid
    #64657
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf114:JMC099-C04:C232626 (SPOUT1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    May 15, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. IRE1b.del-C.9E10.pCDNA3

    Plasmid
    #21882
    Depositor
    David Ron
    Insert
    IRE1b (Ern2 Mouse)
    Use
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    delta C-term
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21882-plasmid-map-sequence-id-10063

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  8. XE184 rat Frizzled-2-myc-pCD

    Plasmid
    #16812
    Depositor
    Randall Moon
    Insert
    frizzled-2 (Fzd2 Rat)
    Use
    Xenopus expression
    Tags
    myc
    Expression
    Mutation
    Promoter
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only

  9. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  10. pETM-30-GST-Dmp68_B

    Plasmid
    #145962
    Purpose
    Bacterial Expression of Dmp68
    Depositor
    Elisa Izaurralde
    Insert
    Dmp68 (Rm62 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145962-plasmid-map-sequence-id-382819

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  11. 4TVR

    Plasmid
    #61952
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBH14 (UHRF2 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. lox2272 Neo* lox2272

    Plasmid
    #168769
    Purpose
    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168769-plasmid-map-sequence-id-337468

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  13. pDEST51-LRRK2-I1371V

    Plasmid
    #29399
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Ile1371Val
    Promoter
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. pIC235

    Plasmid
    #69738
    Purpose
    Retroviral expression of CENP-R
    Depositor
    Iain Cheeseman
    Insert
    CENP-R (ITGB3BP Human)
    Use
    Retroviral
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  16. integrin beta6 EGFP pWZL Blast2

    Plasmid
    #13591
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 (ITGB6 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  17. pRNATU6.1 HDAC3 shRNA2

    Plasmid
    #112271
    Purpose
    HDAC3 KD
    Depositor
    Simone Di Giovanni
    Insert
    shRNA against mouse HDAC3 (Hdac3 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112271-plasmid-map-sequence-id-213643

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  18. pFlagCMV-mCALEBshort

    Plasmid
    #89398
    Purpose
    mammalian expression of mouse CSPG5 short isoform
    Depositor
    Fritz Rathjen
    Insert
    CSPG5 (Cspg5 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    short isoform
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89398-plasmid-map-sequence-id-177323

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  19. SK-hPar3

    Plasmid
    #53229
    Purpose
    bacterial expression of human Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2RL2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53205
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and kanMX6
    Expression
    Mutation
    Promoter
    Gal1 promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pcDNA6-chCAR-MycHis

    Plasmid
    #87232
    Purpose
    Mammalian expression of chicken CAR
    Depositor
    Fritz Rathjen
    Insert
    CXADR (CXADR Chicken)
    Use
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87232-plasmid-map-sequence-id-172951

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  23. pT7-7 asyn S129I

    Plasmid
    #36061
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129I (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S129I
    Promoter
    T7
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36061-plasmid-map-sequence-id-44517

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  24. pE-SUMO CRYBA2

    Plasmid
    #80746
    Purpose
    Expression of human beta A2-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBA2 (CRYBA2 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80746-plasmid-map-sequence-id-153530

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  25. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid
    #147163
    Purpose
    Insect Expression of DmBicaudalC
    Depositor
    Elisa Izaurralde
    Insert
    DmBicaudalC (BicC Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    12 silent and three non silent mutattions I331T, …
    Promoter
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147163-plasmid-map-sequence-id-355687

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  26. pAc5.1B-EGFP-DmPasilla_B

    Plasmid
    #145923
    Purpose
    Insect Expression of DmPasilla
    Depositor
    Elisa Izaurralde
    Insert
    DmPasilla (ps Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    inserted Q66, mutation K77Q, and deletion of AA 9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145923-plasmid-map-sequence-id-363184

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  27. EGFP-hPMCA4bct120

    Plasmid
    #47593
    Depositor
    Emanuel Strehler
    Insert
    PMCA4bct120 (ATP2B4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    120 C terminal AA deleted (constitutively active …
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. IDG_CACNG6_OE_1

    Plasmid
    #161696
    Purpose
    Overexpresses CACNG6
    Depositor
    Mike McManus
    Insert
    CACNG6 (CACNG6 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161696-plasmid-map-sequence-id-334570

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  29. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  30. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  31. pDEST51-LRRK2-T1343G

    Plasmid
    #29400
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Thr1343Gly
    Promoter
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. 6PDM

    Plasmid
    #162264
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRMT9 (PRMT9 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162264-plasmid-map-sequence-id-319823

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  33. pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer

    Plasmid
    #58837
    Purpose
    Expresses GFP-Tagged Human PLC delta PH domain in yeast
    Depositor
    Tim Levine
    Insert
    2x Phospolipase C delta1 PH domain (Plcd1 Rat)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Available since
    Sept. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pTJK637

    Plasmid
    #138010
    Purpose
    GATA1 knockout
    Depositor
    Tami Kingsbury
    Insert
    GATA1 sgRNA (GATA1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138010-plasmid-map-sequence-id-275012

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  36. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. pSMPUW_8.4_Ex_CAMKIIA-EGFP-V5-SV40-Zeo

    Plasmid
    #128464
    Purpose
    lentiviral expression vector with CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128464-plasmid-map-sequence-id-256447

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  39. pQCXIpuro-ITGA5

    Plasmid
    #134764
    Purpose
    a retroviral vector to express integrin alpha5
    Depositor
    Philippe Clezardin, Martine Croset
    Insert
    ITGA5 (ITGA5 )
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    134764-plasmid-map-sequence-id-264380

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  40. p(+)MV-NSE-FlagrP-3g_haP-M502-3p

    Plasmid
    #58798
    Purpose
    Expresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58798-plasmid-map-sequence-id-91093

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  42. GSK-3beta-his (Xenopus)/pCS2+

    Plasmid
    #16335
    Depositor
    Peter Klein
    Insert
    glycogen synthase kinase-3beta (gsk3b.L Frog)
    Use
    Vertebrate expression
    Tags
    6xHis
    Expression
    Mutation
    Xenopus GSK-3 with 6X his tag
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  43. 5C8H

    Plasmid
    #69969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ORC2:JMC095-D11:C231601 (ORC2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. pET11b-MIF Y99A

    Plasmid
    #149440
    Purpose
    Expression of MIF Y99A in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99A
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149440-plasmid-map-sequence-id-311579

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  45. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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  46. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109060-plasmid-map-sequence-id-209546

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  47. pBS chick LH-2A [TJ#93]

    Plasmid
    #16280
    Depositor
    Thomas Jessell
    Insert
    LH-2A (LHX2 Chicken)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 4, 2009
    Availability
    Academic Institutions and Nonprofits only

  48. 4Z30

    Plasmid
    #69957
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H2:YTC020-E12:C234063 (RC3H2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40788-plasmid-map-sequence-id-68904

    Enlarge

  50. pCDNA3-hPXR-FL-T248E

    Plasmid
    #41617
    Depositor
    Sridhar Mani
    Insert
    hPXR-FL-T248E (NR1I2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N10D and T248E
    Promoter
    CMV
    Available since
    March 11, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10801 - 10850 of 52925 results