We narrowed to 26,807 results for: STI

1. pXylA-RgTALsyn

   Plasmid

   #100948

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   XylA (Constitutive)

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pECFP-Cdc42-Y40C

   Plasmid

   #105295

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIII-Neo-E2Crimson

   Plasmid

   #153425

   Purpose

   Retroviral (MSCV) expression of E2-Crimson fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   E2-Crimson
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CyPet-FKBP-E2

   Plasmid

   #175244

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/SPTBN1-FLT3

   Plasmid

   #168115

   Purpose

   Mammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemia

   Depositor

   Nicholas Cross

   Article

   Grand et al Exp Hematol. 2007 Nov;35(11):1723-7. doi

   Insert

   SPTBN1-FLT3
   

   Expression

   Mammalian

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRII-Topo Ptn in situ probe

   Plasmid

   #45632

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Ptn in situ probe (Ptn Mouse)
   

   Use

   In situ

   Mutation

   fragment contains bp# 1241-1830 of NM_008973

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCMV5 Flag Jnk1a1(TPF)

   Plasmid

   #15741

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1(APY) (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Tyr-192Phe

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pLentiCRISPR sgSLC19A1

   Plasmid

   #102314

   Purpose

   genetic depletion of SLC19A1

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgSLC19A1 (SLC19A1 Human)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-term Hec1-targeted Aurora B FRET sensor

   Plasmid

   #45239

   Purpose

   sensor targeted to N-terminus of Hec1

   Depositor

   Michael Lampson

   Article

   Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

   Insert

   Hec1, Aurora B FRET sensor
   

   Tags

   Aurora B FRET sensor and Hec1

   Expression

   Mammalian

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHMWGFPR

    Plasmid

    #64396

    Purpose

    High molecular weight glutenin gene promoter driving GFP expression

    Depositor

    Claudia Vickers

    Article

    Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

    Insert

    High molecular weight glutenin gene promoter
    

    Tags

    GFP

    Expression

    Plant

    Promoter

    High molecular weight glutenin gene promoter

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGBDU-Atg17

    Plasmid

    #121394

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Expression

    Yeast

    Promoter

    ADH1 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA_GFP_mTLN1-FL-LBD

    Plasmid

    #164839

    Purpose

    Expression of GFP tagged FL mouse talin1 mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN1 (Tln1 Mouse)
    

    Tags

    His8-GFP

    Expression

    Mammalian

    Mutation

    Lipid binding deficient mutations

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPQD1CB0108

    Plasmid

    #177041

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPQD1CB0817

    Plasmid

    #177039

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

    Plasmid

    #171726

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

    Plasmid

    #171734

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

    Plasmid

    #171735

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

    Plasmid

    #171727

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

    Plasmid

    #171731

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

    Plasmid

    #171730

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p21 promoter C

    Plasmid

    #16464

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pPyCAG-NE-EGFP-IRES-Pac

    Plasmid

    #183617

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

    Insert

    EGFP
    

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pUASTLOTattB_vhhGFP4::Vkg::mCherry

    Plasmid

    #163929

    Purpose

    Expression of GrabFP-ECM under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    G1767D

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDEST51-LRRK2-R1441H

    Plasmid

    #25083

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arg 1441 mutated to His

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pME-APEX2-mCherry

    Plasmid

    #188944

    Purpose

    Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    mito-APEX2_p2A_APEX2-H2B_p2A_mCherry
    

    Use

    Middle entry vector for gateway (l1-l2)

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJL2

    Plasmid

    #184130

    Purpose

    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pmrp-1

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJL1

    Plasmid

    #184129

    Purpose

    For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pmrp-1 (3kb)

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX4T2-Snf1-cat

    Plasmid

    #52683

    Purpose

    expression of the Snf1 kinase domain in bacteria

    Depositor

    Benjamin Turk

    Article

    Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

    Insert

    SNF1 (SNF1 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes kinase domain only, aa 41-315

    Promoter

    tac

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

30. GST-TEVcs-ATG101

    Plasmid

    #171414

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG101 (ATG101 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ZE43 Dapper-2 pCS2P+

    Plasmid

    #17084

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dapper-2 (dact2 Zebrafish)
    

    Use

    Zebrafish expression

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

32. pET-28a-His6-SUMO-VAMP8

    Plasmid

    #135553

    Purpose

    Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP8
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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33. pAAV-OTp-hM3Dq-Myc

    Plasmid

    #184753

    Purpose

    To drive hM3D fused with Myc tag under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

    Insert

    hM3D (Gq)
    

    Use

    AAV

    Tags

    3xMyc

    Promoter

    mouse Oxytocin promoter

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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34. p21 promoter B

    Plasmid

    #16463

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

35. p21 promoter G

    Plasmid

    #16468

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

36. CMV-Cx35-Halo-C-in

    Plasmid

    #71511

    Purpose

    Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

    Depositor

    John O'Brien

    Article

    Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

    Insert

    Connexin 35
    

    Tags

    HaloTag (internal to sequence)

    Expression

    Mammalian

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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37. pEPQD1CB0119

    Plasmid

    #177051

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    major latex protein-like (NmMLPL) from Nepeta mussinii
    

    Use

    Synthetic Biology

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BII-ChBtW-eSpCas9

    Plasmid

    #133370

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET29a-hNABP2-N

    Plasmid

    #128308

    Purpose

    Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    hNABP2 N terminal fragment (OB fold) (NABP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal domain (1–138 aa)

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-EGFP-DmCup_C

    Plasmid

    #146041

    Purpose

    Insect Expression of DmCup

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmCup (cup Fly)
    

    Expression

    Insect

    Mutation

    P418S

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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