We narrowed to 24,259 results for: Nes
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Plasmid#112713PurposeFor tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.DepositorInsertRngtt (Rngtt Mouse)
UseTagsBirA* (BirA R118G), HIV Rev nuclear localization …ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pETM33_Nsp3c_SUD-N
Plasmid#156469PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-M
Plasmid#156470PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(TBK1)
Plasmid#109322PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1DepositorInsertmCherry-KASH (TBK1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-CGG-BsmBI
Plasmid#222966Purpose0CGG with BsmBI site for inserting CGG repeatsDepositorInsertFMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTR, BsmBI site …PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-dCGG0
Plasmid#222965PurposeTo create 0CGG FMR1 cell lineDepositorInsertFMR1 homologous template no CGG repeats (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTRPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro 2xStrep-HPC4-Gb5
Plasmid#218177PurposeUsed to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cellsDepositorInsertG beta subunit 5 (GNB5 Human)
UseLentiviralTags2x Strep tag and HPC4ExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-tTA
Plasmid#178516PurposeAAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expressionDepositorInserttTA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-jGCaMP7s
Plasmid#178514PurposeAAV vector for Flpo recombinase dependent jGCaMP7sDepositorInsertjGCaMP7s
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-mCre
Plasmid#178515PurposeAAV vector for mCre recombinaseDepositorInsertmCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-cCE
Plasmid#112711PurposeFor tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsHIV Rev nuclear localization signal (NES), Stop c…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMV with Tet repressor binding sitesAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TTBG
Plasmid#115232PurposeLentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2DepositorInsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TTBL
Plasmid#115233PurposeLentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2DepositorInsertmTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-Flpo
Plasmid#174378Purposealso known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinaseDepositorHas ServiceAAV RetrogradeInsertFLPo recombinase
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQY527A
Plasmid#166949PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
UbC-Halo-SFPQY527A
Plasmid#166947PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromoterUbCAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
KLC1N343S-Myc
Plasmid#166963PurposeExpresses Myc-tagged KLC1 N343S protein in pcDNA3.1DepositorInsertKLC1-N343S (Klc1 Mouse)
UseTagsMycExpressionMammalianMutationKLC1-N343SPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-C
Plasmid#156471PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-C (ORF1ab Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3e_NAB
Plasmid#156473PurposeBacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tagDepositorInsertNsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE
Plasmid#139096PurposeExpresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dxCas9 3.7
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DLG3_PDZ_2
Plasmid#103947PurposeProtein expression and purification of NE-DLG PDZ2 domainDepositorInsertDLG3 (DLG3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hACE2-hygro
Plasmid#161758PurposeExpresses human ACE2 in mammalian cellsDepositorInsertHuman ACE2 (ACE2 Human)
UseLentiviralTagsP2AExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1m
UseAAVTagsExpressionMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK-hACE2
Plasmid#161612PurposeExpression of human ACE2 in mammalian cellsDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR
Plasmid#181973PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR
Plasmid#181972PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SBC009876
Plasmid#226283PurposeExpresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.DepositorInsertsConiferyl alcohol acyltransferase
Eugenol synthase 1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A tail only
Plasmid#166956PurposeExpresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (820-1027aa)PromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S293A
Plasmid#115197PurposeLentiviral transduction and expression of PDHA2S293A into any mammalian cellDepositorInsertPDHA2S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S293APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A delta Tail
Plasmid#166955PurposeExpresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (1-820aa)PromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(TARDBP)
Plasmid#109323PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBPDepositorInsertmCherry-KASH (TARDBP Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2
Plasmid#115192PurposeLentiviral transduction and expression of PDHA2 into any mammalian cellDepositorInsertPDHA2WT (PDHA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A
Plasmid#115196PurposeLentiviral transduction and expression of PDHA2S291A into any mammalian cellDepositorInsertPDHA2S291A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-KLC1 delta Tail
Plasmid#166965PurposeExpresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1DepositorInsertKLC1 (Klc1 Mouse)
UseTagsMycExpressionMammalianMutationKLC1 (1-495aa)PromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5AR280H
Plasmid#166957PurposeExpresses FLAG-tagged KIF5A R280H protein in pcDNA3.1DepositorInsertKIF5A-R280H (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A-R280HPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR
Plasmid#181974PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
UseLentiviralTagsExpressionMutationNGFR truncation: aa 11-276PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-Flag-CDK8-WT-IRES-mCherry
Plasmid#68856PurposeExpresses Human CDK8 Wild-TypeDepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR
Plasmid#181975PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
UseLentiviralTagsExpressionMutationNGFR truncation: aa 11-276PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-W105M-IRES-mCherry
Plasmid#68857PurposeExpresses Human CDK8 W105M MutantDepositorInsertCyclin-Dependent Kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT
Plasmid#128551PurposeLentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-KIF5A-HA-IRES-Puro
Plasmid#166952PurposeLentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoterDepositorInsertKIF5A (Kif5a Mouse)
UseLentiviralTagsHAExpressionMutationPromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(SOD1)
Plasmid#109321PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1DepositorInsertmCherry-KASH (SOD1 Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7H126A
Plasmid#115180PurposeGateway cloning of PARK7H126A into any destination vectorDepositorInsertPARK7H126A (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.H126APromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7E163K
Plasmid#115181PurposeGateway cloning of PARK7E163K into any destination vectorDepositorInsertPARK7E163K (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.E163KPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A/S293A
Plasmid#115198PurposeLentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cellDepositorInsertPDHA2S291A/S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291A/S293APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7V51G
Plasmid#115178PurposeGateway cloning of PARK7V51G into any destination vectorDepositorInsertPARK7V51G (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.V51GPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7C53A
Plasmid#115179PurposeGateway cloning of PARK7C53G into any destination vectorDepositorInsertPARK7C53A (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.C53APromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only