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pET21a.MJ1247

Plasmid

#11414

Depositor

Sung-Hou Kim

Article

Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

Insert

3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)

Tags

T7-tag

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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V954 - pDNR MCS SA no SA CmR - Donor

Plasmid

#11697

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Plasmid

#15629

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
GST-tag RNF14 binding E2 variant L3R14-2

Plasmid

#248850

Purpose

N-term GST tag E2 variant selected for RNF14

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3R14-2

Tags

GST

Expression

Bacterial

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag CUL9 binding E2 variant L3C9-1

Plasmid

#248851

Purpose

N-term GST tag E2 variant selected for CUL9

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3C9-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ANKIB1 binding E2 variant L3AN-1

Plasmid

#248852

Purpose

N-term GST tag E2 variant selected for ANKIB1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3AN-1

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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GST-tag ANKIB1 binding E2 variant L3AN-2

Plasmid

#248853

Purpose

N-term GST tag E2 variant selected for ANKIB1

Depositor

Brenda Schulman

Article

Du et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

GST-L3AN-2

Tags

GST

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pART27

Plasmid

#247986

Purpose

Binary vector to receive a NotI fragment from pART7 with a gene to be stably expressed in plants via agrobacterium-mediated transformation.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(h)-DEST-mKOk

Plasmid

#224830

Purpose

Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Type

Empty backbone

Tags

mKusabiraOrangek

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--Pconst-YFP

Plasmid

#228099

Purpose

recA promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--Pconst-YFP

Plasmid

#228100

Purpose

constitutive promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PrecA-YFP

Plasmid

#228101

Purpose

constitutive promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PgadX-YFP

Plasmid

#228102

Purpose

constitutive promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PrecA-YFP

Plasmid

#228094

Purpose

gadX promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--PgadX-YFP

Plasmid

#228095

Purpose

gadX promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PgadX-CFP--Pconst-YFP

Plasmid

#228096

Purpose

gadX promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

gadX & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PgadX-YFP

Plasmid

#228098

Purpose

recA promoter driving expression of CFP reporter and gadX promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & gadX

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA516

Plasmid

#231118

Purpose

Fragmid fragment: (2A Selection) CBE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ248

Plasmid

#217494

Purpose

Mammlian expression of DpFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc guide RNA scaffold

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJD1

Plasmid

#222374

Purpose

Recombinant human IgG1 antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF125

Plasmid

#222373

Purpose

Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG (partial)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ234

Plasmid

#217492

Purpose

Mammlian expression of DpFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pATT-Dest-Cre

Plasmid

#202714

Purpose

Cre recombinase staging plasmid to make transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

Cre

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27B3)_FLAG

Plasmid

#199613

Purpose

Encodes DARPin-FLAG 27B3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27B3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ E3_5 _hFc

Plasmid

#199617

Purpose

Encodes DARPin-hFc E3_5 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc E3_5 (control)

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCdKN0011

Plasmid

#196152

Purpose

Gateway destination vector for protein expression of 9xHis_AtDREB26_HiBit

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

9xHis_AtDREB26_HiBit

Expression

Bacterial

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0011

Plasmid

#196145

Purpose

Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtDREB26_HiBit

Use

Gateway entry vector

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179672

Purpose

Membrane-bound (Lyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP

Plasmid

#179647

Purpose

HCVP-inducible p450 SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP

Plasmid

#179648

Purpose

TVMVP-inducible single TM SEAP RELEASE construct with SpyTag

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX

Plasmid

#179662

Purpose

Membrane-bound (CAAX) TEVP-inducible caged-nTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged-nTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP

Plasmid

#179643

Purpose

OR Gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD21_035

Plasmid

#109152

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
EGFP

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CMV and pTet

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG369-lyn11-redFAST

Plasmid

#160356

Purpose

Expresses Lyn11-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG361-lyn11-greenFAST

Plasmid

#160352

Purpose

Expresses Lyn11-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

lyn11-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOEM1_pCMV:axFgf8-pPH:vsvged

Plasmid

#111159

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Nacu et al Nature. 2016 May 19;533(7603):407-10. do

Insert

axFgf8

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Olfr16

Plasmid

#105486

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr16 (Olfr16, MOR267-13 Mouse)

Use

To ganerate riboprobe

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Olfr62

Plasmid

#105490

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr62 (Olfr62, MOR258-5 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Olfr54

Plasmid

#105488

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr54 (Olfr54, MOR126-1 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Olfr3

Plasmid

#105487

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Olfr3 (Olfr3, MOR136-14 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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