Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,273 results for: nls

Clear keywords
Showing: 1051 - 1100 of 6273 results

  5. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

    Plasmid
    #47453
    Purpose
    epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (N136,Grm2,C63)-cib1
    Use
    AAV; Tale
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47453-plasmid-map-sequence-id-72982

    Enlarge

  14. RPL25NLS-GFP-PRA (102)

    Plasmid
    #24041
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Tags
    EGFP and PRA
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid
    #172195
    Purpose
    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    172195-plasmid-map-sequence-id-343564

    Enlarge

  20. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid
    #89743
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi dark-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89743-plasmid-map-sequence-id-192503

    Enlarge

  21. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid
    #89742
    Purpose
    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    NLS-OptoJNKi lit-state mutant
    Use
    Fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available Since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89742-plasmid-map-sequence-id-192502

    Enlarge

  22. mPA-GFP-PCNA-19-SV40NLS-4

    Plasmid
    #57154
    Purpose
    Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid
    #85821
    Purpose
    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    Zf Ubi::cMyc oDam_f_GFPoNLS
    Use
    Tol2 based vector
    Tags
    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85821-plasmid-map-sequence-id-170479

    Enlarge

  30. PHO4NLS-GFP-PRA (109)

    Plasmid
    #24044
    Depositor
    Michael Rout
    Insert
    PHO4
    Tags
    EGFP and PrA
    Expression
    Yeast
    Mutation
    Only bp 421-588 of PHO4 (NLS)
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  32. NAB2NLS-GFP-PRA (107)

    Plasmid
    #24043
    Depositor
    Michael Rout
    Insert
    NAB2
    Tags
    EGFP and PrA
    Expression
    Yeast
    Mutation
    Only bp 601-750 of NAB2 (NLS)
    Available Since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  34. His-LAP-BFP-NLS

    Plasmid
    #248826
    Purpose
    LAP2 fused to BFP and NLS, a nuclear localization signal. Imaging shows primarily nucleolar localization.
    Depositor
    Alice Ting
    Insert
    LplA
    Tags
    6xHis and BFP-3xNLS
    Expression
    Mammalian
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248826-plasmid-map-sequence-id-495662

    Enlarge

  43. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid
    #227084
    Purpose
    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    STREPii-FLAG and SV40 NLS 1
    Expression
    Yeast
    Promoter
    Gal1-10
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227084-plasmid-map-sequence-id-451056

    Enlarge

  48. pHR-AL-mCh-Cry2WT-NLS

    Plasmid
    #221924
    Purpose
    Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Daniel Bose
    Insert
    CBP AL (aa 1529-1607) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the autoregulatory loop (AL) (aa 152…
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221924-plasmid-map-sequence-id-437707

    Enlarge

Showing: 1051 - 1100 of 6273 results