We narrowed to 7,655 results for: Pol
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Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
TagsGal8 is fused to the c-terminus of GFPExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1 human cofilin S3A
Plasmid#50860Purposeexpresses constituvely active cofilin fused to EGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcofilin 1 S3A (CFL1 Human)
TagsEGFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-Golgi eGFP
Plasmid#79809PurposeTo express and target GFP into the Golgi apparatus using lentivirusesDepositorInsertGolgi eGFP (B4GALT1 Human)
UseLentiviralTagsFusion of the first 62 amino acids of the human b…ExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCluc, Gluc
UseLuciferaseAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEAK-Flag/INPP4B
Plasmid#24324DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
TagsFlag and HAExpressionMammalianMutationnoneAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phGluc
Plasmid#22522DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGaussia luciferase
ExpressionMammalianAvailable sinceDec. 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGFAP-Cre
Plasmid#40591PurposeMouse astrocyte expression of CreDepositorPublicationInsertCre recombinase (GFAP Human, Mouse, Phage)
UseCre/Lox and Mouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionMammalianMutationConstruct contains a nuclear-targeted Cre recombi…PromoterhGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
ExpressionMammalianAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-NICD
Plasmid#26891DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNICD (Notch1 Mouse)
TagsFLAG and b-globin polyAExpressionMammalianMutationE2299KAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF5-FRT-TagRFP-T-IFT88
Plasmid#61684PurposeMammalian expression of IFT88 fused with N terminal TagRFP.T tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIFT88 (Ift88 Mouse)
TagsTagRFP.TExpressionMammalianPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRExpressionYeastAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRcCMV myc-Plk1 T210D (Nigg LA183)
Plasmid#41158DepositorPublicationAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL2B -1538/+64
Plasmid#24942DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL-10 promoter (Il10 Mouse)
UseLuciferaseTagsLuciferaseAvailable sinceAug. 18, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
TagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-OT (wt TCF-4 sites)
Plasmid#16558DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTCF-4 binding sites (TCF4 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutation3 copies of wildtype Tcf-4 binding sitesAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hPDGFRA-GFP
Plasmid#22919DepositorInserthuman platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
UseAAVExpressionMammalianAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by