We narrowed to 9,127 results for: Pol

1. pcDNA-flag-mKdm4d(H189A)-polyA

   Plasmid

   #61554

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (catalytic mutant)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   changed Histidine 189 (of Kdm4d) to Alanine

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET empty polycistronic destination vector (2Z)

   Plasmid

   #29776

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-Flag-Polß(WT)-IRES-puro

   Plasmid

   #128653

   Purpose

   mammalian expression of Flag-Polß(WT) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124542

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-FHC-POLQ-S1977P

   Plasmid

   #64876

   Purpose

   lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   S1977P, mimicking the chaos1 mutation

   Promoter

   EF1alpha

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-TgKI-MCS-mLanYFP-polyA

   Plasmid

   #174021

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   mLanYFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp128-P30-His-pol30(K164R)

    Plasmid

    #99544

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

    Plasmid

    #156163

    Purpose

    Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated THBS1 (amino acid residues 1-954)

    Promoter

    CMV

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124546

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. attB-mCh-Dnmt3b1-Poly(A)-PuroR

    Plasmid

    #65556

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

    Plasmid

    #159487

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)
    

    Expression

    Mammalian

    Promoter

    U6-27

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB-mCh-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65554

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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