165,307 results

1. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-hPIASx beta

   Plasmid

   #15210

   Depositor

   Ke Shuai

   Article

   Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

   Insert

   PIASx beta (PIAS2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-tTA

   Plasmid

   #104102

   Purpose

   Expresses tTA2 under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIN-PAmCherry-KFERQ-NE

   Plasmid

   #102365

   Purpose

   Lentiviral overexpression of PA-mCherry-KFERQ fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   KFERQ peptide
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV mGAD65(delE1) GFP WPRE SV40pA

   Plasmid

   #177316

   Purpose

   To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

   Depositor

   Hirokazu Hirai

   Article

   Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

   Insert

   mGAD65(delE1) promoter, GABAergic interneurons specific promoter
   

   Use

   AAV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-AMBRA1-3xFLAG

   Plasmid

   #172605

   Purpose

   Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ApoE3

    Plasmid

    #197147

    Purpose

    Expresses ApoE3 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE3 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZP06C

    Plasmid

    #232322

    Purpose

    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

    Depositor

    Chenggang Wu

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    rpsJ

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA1-Tat

    Plasmid

    #138478

    Purpose

    Expresses HIV Tat for efficient sgRNA packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Tat
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Tags

    His-thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB_TRE3G_scFV-sfGFP-Ezh2FL

    Plasmid

    #235575

    Purpose

    Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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