58,344 results

1. TFORF0930

   Plasmid

   #143242

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF83 (ZNF83 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUGW-APPSwe-T2A-mCherry

   Plasmid

   #190804

   Purpose

   Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Article

   Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

   Insert

   APP (APP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Swedish (KM670/671NL)

   Promoter

   Ubi C

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IB-TMEM192-mKeima

   Plasmid

   #215359

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BRD4

   Plasmid

   #38943

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-GRAM-W

   Plasmid

   #211707

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AcGFP-MSH3

   Plasmid

   #215122

   Purpose

   Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   MSH3 (MSH3 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-PIPK1 gamma 90

   Plasmid

   #22299

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-APP-mGFP

   Plasmid

   #196696

   Purpose

   Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   HA and mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSIIpuro-EGF-ScNeo

   Plasmid

   #209893

   Purpose

   To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EGF-ScNeo (EGF Human)
   

   Use

   Lentiviral

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSIIpuro-HBEGF-ScNeo

    Plasmid

    #209894

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ATR Kinase dead

    Plasmid

    #31612

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K2327R (kinase dead)

    Promoter

    Available since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hNPPC-RFP

    Plasmid

    #22921

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E075 Hs.EXOC5

    Plasmid

    #70359

    Purpose

    Gateway ORF clone of human EXOC5 [NM_006544.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    EXOC5 (EXOC5 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Opto-GPR39

    Plasmid

    #106021

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR39; Opto-GPR39; B11)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR39 (GPR39 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDARMO_CMVT_3xFLAG_BIRC6

    Plasmid

    #197967

    Purpose

    Mammalian cell expression of wt 3xFLAG-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L1332V

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23b-Casp3 C163A-His

    Plasmid

    #11822

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 3 catalytic mutant (CASP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C163A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only