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Showing: 4421 - 4440 of 28326 results

  1. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid
    #184636
    Purpose
    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion
    Depositor
    Nicolas Tritsch
    Article
    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:
    Insert
    Gat1 (Slc6a1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Gat1 gene with Arginine 69 changed to Lysine, to …
    Promoter
    EF1a
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184636-plasmid-map-sequence-id-369063

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  4. pN1mcherry-NKD2G2A

    Plasmid
    #174334
    Purpose
    encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    mutation of a.a 2 Gly to Ala
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174334-plasmid-map-sequence-id-349873

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  5. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174333-plasmid-map-sequence-id-349872

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  6. FG11F-C-FLAG-hNKD2

    Plasmid
    #174330
    Purpose
    encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174330-plasmid-map-sequence-id-349820

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  7. MO70-C-FLAG-hNKD2

    Plasmid
    #174327
    Purpose
    encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Retroviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174327-plasmid-map-sequence-id-349813

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  10. pEGFP_∆R13–DD-talin1 (1-2299)

    Plasmid
    #166125
    Purpose
    For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.
    Depositor
    Vesa Hytönen
    Article
    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.
    Insert
    ∆R13–DD-Talin-1 (Tln1 Mouse)
    Use
    Cmv promoter
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    residues 1-2299, deletion of dimerization domain …
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166125-plasmid-map-sequence-id-330294

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  12. Donor-PIGAmut

    Plasmid
    #153548
    Purpose
    Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
    Use
    Donor plasmid
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153548-plasmid-map-sequence-id-319288

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  14. pBB0238-TEV-His12

    Plasmid
    #137038
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137038-plasmid-map-sequence-id-291947

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  17. pSHDY*in_PpetE:BCD2:AgBIS:Flag

    Plasmid
    #133971
    Purpose
    Heterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    AgBIS:Flag
    Use
    Synthetic Biology
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133971-plasmid-map-sequence-id-271350

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Showing: 4421 - 4440 of 28326 results