171,128 results

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pCW57.1-ZNF746-C571A

Plasmid

#207826

Purpose

Dox-inducible expression of ZNF746-C571A

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Mutation

C571A

Promoter

TRE

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Expression

Yeast

Promoter

CUP1 promoter

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pS238D1::sptse5-CT

Plasmid

#192959

Purpose

For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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ZMYND8-2A-neo

Plasmid

#190619

Purpose

Lentiviral expression plasmid of human ZMYND8, neomycin selection

Depositor

Junwei Shi

Article

Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

Insert

ZMYND8 (ZMYND8 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

synonymous mutation for sgRNA resistance at aa214…

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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C413-E40: attB4-EFSp>-attB1r

Plasmid

#162928

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

short form of human EF1 alpha promoter

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPD335 VP64-ZF43(RAAR)

Plasmid

#138819

Purpose

Constitutive expression of VP64-ZF43(RAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(RAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GFP-fGFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2

Plasmid

#40978

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has Service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual LIC cloning vector (5A)

Plasmid

#30121

Depositor

Scott Gradia

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla

Plasmid

#60510

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Article

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 ShhN

Plasmid

#37680

Depositor

Philip Beachy

Insert

shh (Shh Mouse)

Expression

Mammalian

Mutation

soluble form (see comments)

Promoter

CMV

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway

Promoter

alpha-Acrystallin promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.8TM.28.BB.3z

Plasmid

#200672

Purpose

Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEVOL_PylRS(WT)-tRNA

Plasmid

#223512

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

Inserts

Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
tRNA Pyl

Tags

6xHis

Expression

Bacterial

Promoter

araBAD, rrnB, and glnS and proK

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-P4M-SidMx2

Plasmid

#136997

Purpose

Lentiviral EGFP lipid sensor for PI(4)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-P4M-SidMx2

Use

Lentiviral

Tags

EGFP

Promoter

EF1 alpha

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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