We narrowed to 13,928 results for: RING

1. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM1513

   Plasmid

   #108998

   Purpose

   safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Safe haven knock-in plasmid for the act5 locus, n…

   Tags

   GFP

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAG98-mTurquoise2-Nuf2

   Plasmid

   #80760

   Purpose

   Express Nuf2 N-terminally labeled with mTurquoise2

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-bsd-Flag-Trl1

   Plasmid

   #126609

   Purpose

   Expresses N-terminal Flag-tagged yeast Trl1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA ligase 1 (TRL1 Budding Yeast)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR_eGFP_369

   Plasmid

   #111823

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 349-369
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-GRAB_g5-HT2m

   Plasmid

   #208722

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2m
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pL157A-EGFP

   Plasmid

   #68850

   Purpose

   mutated OPTN was inserted into pEGFP-N1 backbone

   Depositor

   Beatrice Yue

   Article

   Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

   Insert

   optineurin (OPTN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L157A, mutation has not been linked to any diseas…

   Promoter

   CMV

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

   Plasmid

   #91932

   Purpose

   Bacterial expression of maize SUMO1a and SCE1b

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   6His-ZmSUMO1a-GG
   ZmSCE1b-Myc
   

   Tags

   6His and Myc

   Expression

   Bacterial

   Mutation

   Truncation of ZmSUMO1a after the diGlycine motif

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-hEFA6B

   Plasmid

   #206413

   Purpose

   Mammalian expression of hEFA6B

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

   Insert

   EFA6B (PSD4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROP_C9_Puro_JAK2

    Plasmid

    #183303

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    JAK2
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJEC581

    Plasmid

    #174369

    Purpose

    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    RFP with PAM rich sequence upstream promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23117

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23119

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 EB1 shRNA #3

    Plasmid

    #37927

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 shRNA #3 (MAPRE1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_KanR neo

    Plasmid

    #167861

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK102

    Plasmid

    #110574

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRK5myc Cdc42 L61

    Plasmid

    #15906

    Depositor

    Alan Hall

    Article

    Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

    Insert

    Cdc42 (placental isoform) (CDC42 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Q61L

    Available Since

    Nov. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. PECAM-H2B-GFP-Hygro

    Plasmid

    #14684

    Depositor

    Victoria Bautch

    Article

    Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

    Insert

    PECAM promoter/enhancer (Pecam1 Mouse)
    

    Tags

    H2B-GFP and PGK-Hygro

    Expression

    Mammalian

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pKMV-VioE

    Plasmid

    #65351

    Purpose

    systhesis of vioE gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioE
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRK5myc Cdc42 N17

    Plasmid

    #15907

    Depositor

    Alan Hall

    Article

    Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

    Insert

    Cdc42 (placental isoform) (CDC42 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T17N

    Available Since

    Nov. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pMOD_A0908

    Plasmid

    #91027

    Purpose

    Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2-P2A-AtCas9
    

    Use

    CRISPR

    Promoter

    EC1.2

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. mCherry_eDHFR_SIM#1

    Plasmid

    #164646

    Purpose

    tag SIM with mCherry and eDHFR

    Depositor

    Huaiying Zhang, Michael Lampson

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    SIM
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJL1-PD-4xDQNAT

    Plasmid

    #128391

    Purpose

    In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Protein D
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDM1515

    Plasmid

    #109000

    Purpose

    safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, c…

    Tags

    GFP

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23107

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

    Plasmid

    #91934

    Purpose

    Bacterial expression of maize SUMO1a and SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d-Myc
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. OMM PKA Reporter

    Plasmid

    #104550

    Purpose

    Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Tom20 - mTurquoise2 - VASP 148-164
    

    Tags

    mTurqouise2

    Expression

    Mammalian

    Promoter

    EF1 alpha

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-Gdf9

    Plasmid

    #163599

    Purpose

    Expresses GDF-9 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf9 (Gdf9 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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