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We narrowed to 21,470 results for: SPR

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Showing: 8881 - 8920 of 21470 results

  1. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  2. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  3. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  4. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  5. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid
    #140205
    Purpose
    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    mCherry
    sgRNA E1
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA and U3
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140205-plasmid-map-sequence-id-310180

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  14. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  15. pHDR-USR-eGFP

    Plasmid
    #208830
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208830-plasmid-map-sequence-id-414896

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  18. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  31. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  32. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

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  35. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Promoter
    hU6, EF-1a
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

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  37. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

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Showing: 8881 - 8920 of 21470 results