We narrowed to 13,117 results for: CRISPR-Cas9

1. phH1-gRNA

   Plasmid

   #53186

   Purpose

   Expresses the S. pyogenes sgRNA from the H1 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human H1

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV GG hUbC-dsRED

   Plasmid

   #84034

   Purpose

   Contains Golden Gate cassette to express up to four gRNAs and dsRED

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pAra and pTet

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTargetF

   Plasmid

   #62226

   Purpose

   Constitutive expression of sgRNA without donor editing template DNA

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pij23119

   Available since

   March 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT39

   Plasmid

   #204289

   Purpose

   For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-EGFP+H3:H26
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAT10

   Plasmid

   #204293

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRB1017

   Plasmid

   #59936

   Purpose

   empty vector for gRNA cloning

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBGK

   Plasmid

   #65796

   Purpose

   Vector for Cas9 mRNA synthesis

   Depositor

   Masato Ohtsuka

   Article

   Harms et al Curr Protoc Hum Genet. 2014 Oct 1;83:15.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sg_shuttle_RFP657

    Plasmid

    #134968

    Purpose

    Lentiviral vector for sgRNA expression

    Depositor

    Beat Bornhauser

    Article

    Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-sgRNA; EFS-RFP657

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH1v1

    Plasmid

    #60244

    Purpose

    gRNA expression by the H1 promoter

    Depositor

    Donald Zack

    Article

    Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMZ283

    Plasmid

    #52224

    Purpose

    Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMB60

    Plasmid

    #47941

    Purpose

    Can be used to generate synthetic guide in vitro from T7 promotor

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    synthetic guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB8622

    Plasmid

    #126912

    Purpose

    Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6>Ebf.774(F+E)

    Plasmid

    #59990

    Purpose

    U6 promoter driving Ebf.774 (F+E) sgRNA

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    Ebf.774 (F+E) sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6>Control(F+E)

    Plasmid

    #60006

    Purpose

    U6 promoter driving control (F+E) sgRNA

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    Control (F+E) sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. psBIG1a ActBT2AmcherryP2AP -TagBFPH

    Plasmid

    #229971

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2AmcherryP2AP -TagBFPH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-dSa VP64 Afp

    Plasmid

    #99697

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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