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We narrowed to 14,439 results for: eng

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Showing: 4441 - 4460 of 14439 results

  4. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid
    #98218
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98218-plasmid-map-sequence-id-256051

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  5. pcDNA3-Lyso-Digda

    Plasmid
    #236102
    Purpose
    Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyso-Digda (LAMP1 Human, Synthetic)
    Tags
    Full-length LAMP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236102-plasmid-map-sequence-id-473791

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  6. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  7. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid
    #205151
    Purpose
    Express UbC-driven LARGE1 and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205151-plasmid-map-sequence-id-405996

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  8. DNMT3L-dCas9

    Plasmid
    #126587
    Purpose
    DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3L (DNMT3L Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3L-dCas9-NLS
    Expression
    Mammalian
    Available since
    Dec. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    126587-plasmid-map-sequence-id-244969

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  16. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid
    #132962
    Purpose
    Firefly-luciferase single-strand annealing assay cloning backbone
    Depositor
    Gang Bao
    Publication
    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly Luciferase
    Use
    CRISPR and Luciferase
    Expression
    Mammalian
    Mutation
    Stop sequence between luciferase gene
    Promoter
    CMV
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132962-plasmid-map-sequence-id-259217

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  19. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Publication
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    179472-plasmid-map-sequence-id-375147

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  20. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Publication
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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Showing: 4441 - 4460 of 14439 results