We narrowed to 12,578 results for: LIC

1. pET21d-colEdes17/Imdes17

   Plasmid

   #107125

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes17/Imdes17
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21d-colEdes18/Imdes18

   Plasmid

   #107126

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes18/Imdes18
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21d-colEdes1/Imdes1

   Plasmid

   #107108

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes1/Imdes1
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21d-colEdes2/Imdes2

   Plasmid

   #107109

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes2/Imdes2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21d-colEdes3/Imdes3

   Plasmid

   #107110

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes3/Imdes3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21d-colEdes3.5/Imdes3.5

   Plasmid

   #107111

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes3.5/Imdes3.5
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21d-colEdes4/Imdes4

   Plasmid

   #107112

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes4/Imdes4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21d-colEdes5/Imdes5

   Plasmid

   #107113

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes5/Imdes5
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21d-colEdes6/Imdes6

   Plasmid

   #107114

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes6/Imdes6
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD864-LacZ backbone

    Plasmid

    #109384

    Purpose

    Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p415 δCLD

    Plasmid

    #73876

    Purpose

    δCLD - His tagged (2α helices+μHD deleted)

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    δCLD
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P415 Ret2LD

    Plasmid

    #73875

    Purpose

    Ret2LD - His tagged (2α helices+μHD deleted)

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Rat NCKX2-AS-long (pcDNA3.1+)

    Plasmid

    #75196

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Tsoi et al J Biol Chem. 1998 Feb 13;273(7):4155-62.

    Insert

    SLC24A2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Rat NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75197

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Tsoi et al J Biol Chem. 1998 Feb 13;273(7):4155-62.

    Insert

    SLC24A2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Mouse NCKX4-AS-short (pcDNA3.1+)

    Plasmid

    #75207

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (Slc24a4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon absent

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMPO 62

    Plasmid

    #69870

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII- sspH2 signal peptide, M13 replicat

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMPO 60

    Plasmid

    #69868

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF attenuator-MCSII, M13 replication origin

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMPO 55

    Plasmid

    #69866

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII, M13 replication origin

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMPO 59

    Plasmid

    #68364

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication origin

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMPO 52

    Plasmid

    #68359

    Purpose

    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII, ColE1 replication origin

    Depositor

    Eduardo Santero

    Article

    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PrbC5

    Plasmid

    #24787

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    Prb
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

22. BS.YxiN.Twin1.a1-368.wt

    Plasmid

    #13528

    Depositor

    David McKay

    Article

    Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

    Insert

    B. subtilis DEAD-box helicase YxiN first two domains
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    deleted amino acid residues 369-479

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BS.YxiN.Twin1.a404-479.wt

    Plasmid

    #13529

    Depositor

    David McKay

    Article

    Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

    Insert

    B. subtilis DEAD-box helicase YxiN specific RNA binding domain
    

    Use

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    delete amino acid residues 1-403

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xHA-TurboID-NLS_pCDNA3

    Plasmid

    #107171

    Purpose

    expresses 3xHA-tagged TurboID in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-anti-mCherry(LaM6) PAGER(Gq)

    Plasmid

    #230016

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3xHA-miniTurbo-NLS_pCDNA3

    Plasmid

    #107172

    Purpose

    expresses 3xHA-tagged miniTurbo in the mammalian nucleus

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. APEX2-V5-G3BP1_pTRE-G418

    Plasmid

    #202013

    Purpose

    expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    APEX2-V5-G3BP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209726

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

    Plasmid

    #209727

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LD-B

    Plasmid

    #209861

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. His6-MBP-uTEV3

    Plasmid

    #135464

    Purpose

    Bacterial expression of uTEV3 (full-length) protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    His6-MBP-uTEV3
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability) , I138T/S153N…

    Promoter

    T5

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDH-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230018

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gi) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM4D
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

    Plasmid

    #209728

    Purpose

    Expresses cytosolic high affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH-SFFV-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230021

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gi) in human T cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM4D
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLX304 CMV FKBP-V5-sTurboID (N)

    Plasmid

    #153002

    Purpose

    Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

    Plasmid

    #153003

    Purpose

    Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MCS-BioID2-HA

    Plasmid

    #74224

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LD-GA

    Plasmid

    #209862

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Lyso-GA

    Plasmid

    #209874

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. V5-miniTurbo-NES_pCDNA3

    Plasmid

    #107170

    Purpose

    expresses V5-tagged miniTurbo in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Cav-GA

    Plasmid

    #209880

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    CAVIN1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge