We narrowed to 52,941 results for: plasmid

1. pT7-V5-SBP-C1-HsUpf1-V161ER162E-siRNAres_Q

   Plasmid

   #147332

   Purpose

   Mammalian Expression of HsUpf1-V161ER162E

   Depositor

   Elisa Izaurralde

   Insert

   HsUpf1-V161ER162E (UPF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 non silent mutations A69S, E167G, K282R, A442G…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

   Plasmid

   #28289

   Purpose

   3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

   Depositor

   Gus Frangou, Alex Chenchik

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

   Plasmid

   #228636

   Purpose

   Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Enterovirus A71 3C protease isoform B
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. NES-Grx1-roGFP2

   Plasmid

   #236551

   Purpose

   Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   NES-Grx1-roGFP2 (NES Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

   Plasmid

   #228639

   Purpose

   Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Coxsackievirus 33 isoform B 3C protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pRSII416

   Plasmid

   #35456

   Depositor

   Steven Haase

   Article

   Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

   Type

   Empty backbone

   Use

   Yeast centromere vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-Casp2-Flag

   Plasmid

   #11811

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 (CASP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Myc-Foxo1 AAA (pCMV5)

   Plasmid

   #17547

   Depositor

   Domenico Accili

   Insert

   Foxo1 AAA (Foxo1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Changed T24A T253A S316A

   Promoter

   CMV

   Available since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pGTVL2-CRBN-TBD

   Plasmid

   #196369

   Purpose

   protein expression of the GST-tagged Cereblon Thalidomide binding domain

   Depositor

   Stefan Knapp

   Insert

   CRBN (CRBN Human)
   

   Use

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   contains only T319-T425

   Promoter

   T7

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRACER LA

    Plasmid

    #15003

    Depositor

    Raymond Birge

    Insert

    LA (MFGE8 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. POLG-pcDNA3.1

    Plasmid

    #191487

    Purpose

    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

    Depositor

    Hans Zempel

    Insert

    POLG (POLG Human)
    

    Use

    Tags

    V5 tag, 6X His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IDG_GPR89A_OE_1

    Plasmid

    #161635

    Purpose

    Overexpresses GPR89A

    Depositor

    Mike McManus

    Insert

    GPR89A (GPR89A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RasV12 in pENTR1A (w99-1)

    Plasmid

    #22252

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Scarlet-Kif5a

    Plasmid

    #118745

    Purpose

    Expresses scarlet tagged Kif5a

    Depositor

    Beverly Koller

    Insert

    Kif5a cDNA (Kif5a Mouse)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pQE80L Hu IL-15-His tag

    Plasmid

    #183256

    Purpose

    Bacterial expression of human IL-15 with N-terminal His tag

    Depositor

    Alexander McLellan

    Insert

    IL-15 (IL15 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRZ

    Plasmid

    #27663

    Depositor

    Graeme Conn

    Article

    Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Type

    Empty backbone

    Use

    Rna transcription

    Tags

    HDV Ribozyme

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KE-005

    Plasmid

    #133440

    Purpose

    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQXCINeo-LOX

    Plasmid

    #134761

    Purpose

    a retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)

    Depositor

    Martine Croset, Philippe Clezardin

    Insert

    LOX (LOX Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUHD Cdk1 WT HA

    Plasmid

    #27652

    Depositor

    Greg Enders

    Insert

    Cdk1 (CDK1 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

22. integrin alpha9 EGFP-N3

    Plasmid

    #13600

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBOB-VE-cadherin-tdTomato

    Plasmid

    #118733

    Purpose

    To express VE-cadherin tagged with tdTomato on C-terminus

    Depositor

    John Cooper

    Insert

    Cadherin 5 (CD144) (CDH5 Human)
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mutation

    I503T (Common SNP-see comments)

    Promoter

    CMV

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid

    #212705

    Purpose

    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

    Depositor

    Elzo de Wit

    Insert

    ZFP143-FKBPV-2HA-P2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Limk1_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64368

    Purpose

    lentiviral expression of Link1 and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Limk1 (LIMK1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pLV-EF1a-V5-Bet1

    Plasmid

    #120243

    Purpose

    Expresses V5-tagged Bet1 in lentiviral vector

    Depositor

    Larry Gerace

    Insert

    Bet1 (Bet1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOET5 rota-VLP

    Plasmid

    #218147

    Purpose

    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).

    Depositor

    Minna Hankaniemi

    Insert

    Rotavirus VP6
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. cacna2d1 pcDNA3

    Plasmid

    #206103

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP Cited2

    Plasmid

    #112246

    Purpose

    expression of EGFP-Cited2 in mammalian cells

    Depositor

    Simone Di Giovanni

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAKlux3

    Plasmid

    #14082

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    lacIq repressor gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. IDG_GRID1_OE_1

    Plasmid

    #161661

    Purpose

    Overexpresses GRID1

    Depositor

    Mike McManus

    Insert

    GRID1 (GRID1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. IDG_KCNK7_OE_1

    Plasmid

    #161663

    Purpose

    Overexpresses KCNK7

    Depositor

    Mike McManus

    Insert

    KCNK7 (KCNK7 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-HA-ADH

    Plasmid

    #11610

    Depositor

    Steven Johnson

    Insert

    aldehyde dehydrogenase (ALDH1A1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K412I

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p2RZ

    Plasmid

    #27664

    Depositor

    Graeme Conn

    Article

    Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Type

    Empty backbone

    Use

    Rna transcription

    Tags

    HDV Ribozyme

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-mouse cdk6 wt

    Plasmid

    #75170

    Purpose

    Mammalian expression of mouse cdk6

    Depositor

    Martine Roussel

    Insert

    cyclin dependent kinase 6 (Cdk6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

36. Banshee RunxDN mCherry

    Plasmid

    #80158

    Purpose

    retroviral expression of Runx1DN and mCherry

    Depositor

    Ellen Rothenberg

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and IRES-mCherry

    Expression

    Mammalian

    Mutation

    DN--truncated version of the Runx1 protein contai…

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLJM1-4EBP1-T37A/T46A

    Plasmid

    #136420

    Purpose

    Stable overexpression of elF4EBP1 T37A/T46A

    Depositor

    Gabriel Leprivier

    Insert

    eIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T37A/T46A

    Promoter

    CMV

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-HA_mouse_Adar2_Tv1

    Plasmid

    #158110

    Purpose

    expresses mADAR2 shortest transcript

    Depositor

    Emanuela Felley-Bosco

    Insert

    mAdar2 (Adarb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hKv2.1-pEGFP-C1

    Plasmid

    #111538

    Purpose

    Expresses human KCNB1 in mammalian cells for imaging

    Depositor

    Federico Sesti

    Insert

    human Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. IDG_GABRP_OE_1

    Plasmid

    #161679

    Purpose

    Overexpresses GABRP

    Depositor

    Mike McManus

    Insert

    GABRP (GABRP Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p406CYC1

    Plasmid

    #15975

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. NET43 pEGFP-N2 (609)

    Plasmid

    #61991

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Insert

    NET43 (NAT10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pBS mFgfr1 (CT#92)

    Plasmid

    #14005

    Depositor

    Cliff Tabin

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

44. pET28a_LYN

    Plasmid

    #224238

    Purpose

    Expression of human LYN Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    lyn (LYN Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRSII415

    Plasmid

    #35454

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast centromere vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pETM-11-6His-DmBelle_B

    Plasmid

    #145950

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRS40H

    Plasmid

    #35479

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSBbi-RP Hu CXCR3

    Plasmid

    #183255

    Purpose

    Expression of human CXCR3 in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

    Plasmid

    #160607

    Purpose

    TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

    Depositor

    Diego Orzaez

    Insert

    CUP2Gal4AD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pRPA32-EGFP

    Plasmid

    #211383

    Purpose

    mammalian expression of human RPA32 C-terminally fused to EGFP

    Depositor

    Helmut Pospiech

    Insert

    RPA32 (RPA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge