We narrowed to 18,681 results for: tec

1. SaKKH-ABEmax

   Plasmid

   #119815

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSB04X

   Plasmid

   #117258

   Purpose

   Genome editing for Lactobacillus brevis ATCC367

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB1619 hU6-BpiI-Cas13bt1-DR

   Plasmid

   #176314

   Purpose

   hU6-BpiI-Cas13bt1-DR (crRNA expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   Cas13bt1 crRNA direct repeat
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBY3_ligD-HygR

   Plasmid

   #104625

   Purpose

   CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

   Depositor

   Sheng Yang

   Article

   Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   FnCpf1 codon-optimized C.glutamicum

   Promoter

   hsp60

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/Pt-mChe

   Plasmid

   #183164

   Purpose

   Construct for transient expression of plastids-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Pt-mCherry
   

   Use

   Tags

   57 aa of the Pisum sativum rubisco small subunit …

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR26 GFP Dest

   Plasmid

   #74283

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BiP-mCherry

   Plasmid

   #62233

   Purpose

   Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

   Depositor

   Erik Snapp

   Article

   Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

   Insert

   hamster BiP-mCherry-KDEL
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL-kasOp-FnCas12a2

   Plasmid

   #177874

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF-HdrB3

   Plasmid

   #184908

   Purpose

   carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cas9-sgRNA-homology arms
   

   Use

   CRISPR; Broad-host-range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115268

    Purpose

    Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    EiCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB4906

    Plasmid

    #106150

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

    Plasmid

    #171436

    Purpose

    Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

    Plasmid

    #191144

    Purpose

    Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. nLbCas12a

    Plasmid

    #213745

    Purpose

    For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    nLbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB EF1a CCR6-eLOV-TEVcs-Gal4

    Plasmid

    #173119

    Purpose

    Expresses CCR6 eLOV receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCR6-eLOV-TEVcs-Gal4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-blueAKAR

    Plasmid

    #118470

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH41-Tier1-PhCMV-GLuc

    Plasmid

    #169544

    Purpose

    Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Gaussia Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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