We narrowed to 21,470 results for: SPR

Showing: 4501 - 4520 of 21470 results

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AsdCas12a KRAB

Plasmid

#188502

Purpose

Expresses FLAG tagged AsdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PJQ49

Plasmid

#134657

Purpose

Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23115

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Lb

Plasmid

#138096

Purpose

Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1-plus

Plasmid

#126768

Purpose

Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; Q695A; Q926A; amino acids 1005-1013 replac…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPV-H1-ccdB-mEF1α-RiH

Plasmid

#100598

Purpose

piggyBac vector for sgRNA cloning

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Type

Empty backbone

Use

Piggybac vector

Expression

Mammalian

Promoter

Human H1 PolIII promoter, human FTH1 promoter, mo…

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-2a-6MTnlstdTomato

Plasmid

#67699

Purpose

3' entry clone with 6xmyc nuclear localized tdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

tdTomato

Tags

2A-6xMyc-NLS

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

U6-sgRNA (recipient)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Expression

Bacterial

Promoter

sacB promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-dCas9-EGFP-HaloTag

Plasmid

#169451

Purpose

expression of dCas9-EGFP-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-EGFP-HaloTag

Use

Lentiviral

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141A2.0

Plasmid

#99896

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSH-CAG-AtAFB2.F74A-IRES-puro

Plasmid

#216245

Purpose

Express AtAFB2.F74A mutant without tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSpG-PPE

Plasmid

#170130

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Insert

nCas9-SpG(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

Promoter

maize Ubiquitin-1

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEntr_PE2

Plasmid

#149548

Purpose

Gateway entry clone with PE2 enzyme (with stop codon)

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR; Gateway shuttle vector

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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