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NES-EGFP-cPHx3

Plasmid

#116855

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Publication

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem trimer)

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Publication

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Publication

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEB2-mScarlet-I

Plasmid

#104007

Purpose

Plasmid encoding mScarlet-I

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

Plasmid

#92139

Purpose

Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)

Use

Mouse Targeting

Expression

Mammalian

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmScarlet_H2A_C1

Plasmid

#85051

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Publication

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORI28

Plasmid

#71595

Purpose

EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

Depositor

Todd Klaenhammer, Jan Kok

Publication

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Type

Empty backbone

Use

Integration vector for bacteria

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Publication

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Uri Alon, Galit Lahav

Publication

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

MDM2 (MDM2 Human)

Tags

EYFP

Expression

Mammalian

Promoter

MDM2

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti.CAG.H2B-Dendra2.W

Plasmid

#51005

Purpose

Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-Dendra2

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

CAG

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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iRFP-P4M-SidM

Plasmid

#51470

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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3XFlagNICD3

Plasmid

#20185

Depositor

Raphael Kopan

Publication

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 3 Intracellular Domain (Notch3 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 3 Intracellular Domain (Met…

Promoter

CMV

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Publication

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PJ-Sac

Plasmid

#38000

Depositor

Robin Irvine

Publication

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1 (SAC1 Budding Yeast)
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Mutation

Asp 1263 mutated to Ala

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti 4.1 Ex miR200b-200a-429

Plasmid

#35533

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Publication

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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