We narrowed to 5,124 results for: PEP

1. GPR97-DuET

   Plasmid

   #213294

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR97 (ADGRG3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniSOG-ER-5

   Plasmid

   #57766

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   ER (CALR Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. GCGR-DuET

   Plasmid

   #213250

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GCGR (GCGR Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PD2529 human beta1 full

   Plasmid

   #221401

   Purpose

   Mammalian expression of human integrin beta1 full-length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 full-length (ITGB1 Human)
   

   Use

   Tags

   CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

   Expression

   Mammalian

   Mutation

   codon-optimized mature sequence

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR87-DuET

   Plasmid

   #213293

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR87 (GPR87 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac1-His10FLAG

   Plasmid

   #157736

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Use

   Tags

   His10 + 3xFLAG, enterokinase-cleavable

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BbsI)_CBh-Cas9-T2A-BFP

   Plasmid

   #64323

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADRB3-DuET

   Plasmid

   #213182

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRB3 (ADRB3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0235

   Plasmid

   #142488

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. APJ-DuET

    Plasmid

    #213185

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    APJ (APLNR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid

    #113949

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPR123-DuET

    Plasmid

    #213262

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR123 (ADGRA1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 Myc2- Hs TTP WT

    Plasmid

    #107008

    Purpose

    Expresses epitope-tagged wild-type human TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (ZFP36 Human)
    

    Use

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

    Plasmid

    #194457

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac1-His10TEV

    Plasmid

    #159427

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Use

    Tags

    TEV-protease-cleavable His10-tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C3AR1-DuET

    Plasmid

    #213191

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    C3AR1 (C3AR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid

    #190597

    Purpose

    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    EGFP - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-FLAG-TAPBPR-TM-VC

    Plasmid

    #135499

    Purpose

    Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal/Extracellular FLAG epitope tag, Signal pe…

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GPR146-DuET

    Plasmid

    #213265

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR146 (GPR146 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge