164,577 results

1. pCEP4-myc-CXCR4

   Plasmid

   #98946

   Purpose

   Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGPTVII-Bar-U-MatryoshCaMP6s

   Plasmid

   #100024

   Purpose

   Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBItetDMPKSGFP

   Plasmid

   #96903

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBItetDT480GFP

   Plasmid

   #96905

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-Z23

   Plasmid

   #120066

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Halo
   

   Use

   Pcr template

   Tags

   Halo

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. KDM4D

   Plasmid

   #73247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KDM4D (KDM4D Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam02375:JmjN 18-64, pfam02373:JmjC 182-295

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. alpha-0 CFP in pcDNA3.1

   Plasmid

   #36396

   Depositor

   Narasimhan Gautam

   Article

   Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

   Insert

   G protein alpha 0 (Gnao1 Rat)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted into the KpnI-SpeI sites engineered …

   Promoter

   cmv

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. FTL

   Plasmid

   #100750

   Purpose

   Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   FTL (FTL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-MPP8_Flag2-Blast

    Plasmid

    #175038

    Purpose

    Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    Mphosph8 (Mphosph8 Mouse)
    

    Use

    Piggybac

    Tags

    Flag2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR223-LMP1

    Plasmid

    #195805

    Purpose

    Gateway Entry Clone of Epstein-Barr virus LMP1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    LMP1 (LMP-1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-perox-tagRFP

    Plasmid

    #185600

    Purpose

    Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    perox-tagRFP-T
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgNT.2#2/Cre

    Plasmid

    #173661

    Purpose

    Expresses a NT.2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid

    #214265

    Purpose

    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

    Depositor

    D. James Surmeier

    Insert

    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Synapsin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAXY0013

    Plasmid

    #205582

    Purpose

    NpuDnaE(C)_HYG(53-341)_RUBY

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

    Insert

    NpuDnaE(C)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINVHH_his_flag

    Plasmid

    #210404

    Purpose

    For expression of VHH with C-terminal his and Flag tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6-flag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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