We narrowed to 18,759 results for: nls
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Plasmid#167877PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Hygro
Plasmid#167859PurposePiggyBac compatible plasmid expressing spCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EBFP2
Plasmid#167857PurposePiggyBac compatible plasmid expressing spCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -0
Plasmid#180010PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterTEF1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_LMNA_G2
Plasmid#178090PurposeExpresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAT15489_dABE8e
Plasmid#174127Purposeplasmid expressing ABE8e with dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15482_ABE8e
Plasmid#174120PurposeExpresses ABE8e with an EF1a promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteABE
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianPromoterEF1-aAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSOX2.1.0-gDNA
Plasmid#176382PurposeExpresses spCas9 and gRNA sequence targeting SOX2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSOX2 gRNA
UseCRISPRAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-NQL005-SOX2-sgRNA
Plasmid#175553PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
UseMouse TargetingExpressionMammalianAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EC2_7_dCas9_HC_sgRNA
Plasmid#163711PurposeHigh copy plasmid with dCas9 and sgRNA expression cassettesDepositorInsertdCas9
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL513
Plasmid#169153PurposeEncodes OptoQ-INVRT1DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL358
Plasmid#169147PurposeEncodes OptoQ-AMP1DepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb2Cas12a
Plasmid#174676PurposeExpresses 6xHis-MBP tagged Mb2Cas12aDepositorPublicationInserthuMb2Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT2Aneo-ERT2CreERT2
Plasmid#173850PurposeEncoding Cre recombinase (activated in response to tamoxifen)DepositorInsertERT2CreERT2
ExpressionMammalianAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
ExpressionInsectAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CVS-N2c-nl.EGFP-jRGECO1a
Plasmid#172386PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + jRGECO1a
UseNeurotropic virusAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-FlpO
Plasmid#172379PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear-localized EGFP + FlpO
UseNeurotropic virusAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_AMBRA1#1
Plasmid#174152PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pM198
Plasmid#173218PurposeExpresses OptGFP(1-9)-GB1DepositorInsertOptGFP(1-9)
UseCRISPR and LentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
TagsMycExpressionInsectPromoterUASAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L545
Plasmid#173024PurposeEncodes OptoEXP2 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L580
Plasmid#173023PurposeEncodes OptoAMP4 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BEACON1
Plasmid#171697PurposeExpresses BEACON1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBEACON1
UseCRISPRExpressionMammalianMutationhAPOBEC3A_W104A/Y132DPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-OptoCAR-mScarlet
Plasmid#170463PurposeExpresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophoreDepositorInsertOptoCAR
UseLentiviralTagsmScarletExpressionMammalianPromoterSFFVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR Src-TagBFP
Plasmid#171036PurposeTagBFP-tagged Src kinaseDepositorInsertSrc
UseLentiviralExpressionMammalianAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU319
Plasmid#167686PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU323
Plasmid#167690PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::Cas9i
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU61
Plasmid#167692PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_B6
Plasmid#170300PurposeA knock-out vector for the mouse Ptgs2 in C57BL6DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dAaCas12b-Act3.0
Plasmid#158413PurposeCRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B WT
Plasmid#171770Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B WTDepositorAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_Z3_Gal4VP64
Plasmid#169916PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiniCAFE
UseCRISPRMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterDpy-30Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2Aneo-tdTomato-CAAX
Plasmid#170284PurposeEncoding membrane-anchored tdTomato.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato-CAAX
ExpressionMammalianAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorPublicationInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only