We narrowed to 9,107 results for: control

1. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

   Plasmid

   #211905

   Purpose

   Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD19-scFv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMZ828

   Plasmid

   #155322

   Purpose

   Constitutive expression of PhyB(1-650)-VP16 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SVA-lncRNA AK057321 shRNA 1S scramble

   Plasmid

   #202837

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   SVA-lncRNA AK057321 shRNA 1S scramble control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   6

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. SVA-lncRNA AK057321 shRNA 2S scramble

   Plasmid

   #202839

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   SVA-lncRNA AK057321 shRNA 2S scramble control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dr-FGFR1

   Plasmid

   #184005

   Purpose

   Dr-FGFR expression in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

   Insert

   Dr-FGFR1 (FGFR1 Human)
   

   Use

   Tags

   Deinococcus radiodurans bacterial phytochrome

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2h70CRET(#325)

   Plasmid

   #184060

   Purpose

   cyclofen-inducible CRE activation in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

   Insert

   CRE-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Hsp70-4

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

   Plasmid

   #182256

   Purpose

   Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

    Plasmid

    #182254

    Purpose

    Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

    Plasmid

    #182253

    Purpose

    Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

    Plasmid

    #182255

    Purpose

    Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBICI_high ori

    Plasmid

    #179273

    Purpose

    Expresses CuCBP and BaSP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDUBI

    Plasmid

    #179274

    Purpose

    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    sucrose phosphorylase

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174646

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    CMV (with TetOn)

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid

    #174635

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

    Plasmid

    #174632

    Purpose

    Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid

    #174627

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-HE-bPAC(R278A)-Myc-eYFP

    Plasmid

    #165488

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    bPAC(R278A)-Myc-eYFP
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    EYFP and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EcRfaHKOW-L1L2NusG

    Plasmid

    #113126

    Purpose

    Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107, replaced L1 and L2 loops …

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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