170,204 results

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV V5-LUC Blast (w567-1)

Plasmid

#21474

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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JUP_pLX307

Plasmid

#98347

Purpose

Lentiviral expression of JUP

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

JUP (JUP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-wt

Plasmid

#12972

Depositor

Gary Bokoch

Insert

Cdc42 (CDC42 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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Pomc-pGL3

Plasmid

#17553

Purpose

Luciferase expression under control of Pomc promoter (–646 to +65).

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Pomc promoter (Pomc Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

Pomc

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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Pet28a_Synthetic_Human_H2AZ.1

Plasmid

#42629

Depositor

Joe Landry

Insert

Human H2AZ.1 (H2AZ1 Human, Codon Optimized, Synthetic)

Tags

Untagged

Expression

Bacterial

Mutation

Codon Optimized

Promoter

T7

Available Since

March 21, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCTCON2-GFP

Plasmid

#158130

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+LuxG13

Plasmid

#26161

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon + G13 promoter

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

H231L

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EKAREN5-gl

Plasmid

#225958

Purpose

The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-gl

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198467

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv 2024.07.11.602973

Insert

10His-pG-Tn5 (E54K, L372P)

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BCL2

Plasmid

#116717

Purpose

Lentiviral expression of BCL2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BCL2 (BCL2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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