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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Promoter

U6

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-ER-3

Plasmid

#55041

Purpose

Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

mCherry

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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eGFPC1 FKBP

Plasmid

#67529

Purpose

Expresses GFP fused FKBP

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Insert

FKBP

Tags

eGFP

Expression

Mammalian

Mutation

F36V

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T1

Plasmid

#80495

Purpose

AAVS1 TALEN expression vector (Left)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Left)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T2

Plasmid

#80496

Purpose

AAVS1 TALEN expression vector (Right)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Right)

Use

Gateway entry

Promoter

none

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstarC

Plasmid

#232737

Purpose

Expresses control sensor NAPstarC in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstarC

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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tetO-ID1

Plasmid

#170692

Purpose

doxycycline-inducible overexpression of ID1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ID1 (ID1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-empty-pgk-hph

Plasmid

#48753

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Neo DEST (705-1)

Plasmid

#17392

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HPH-C4

Plasmid

#160343

Purpose

High-copy expression vector for K. marxianus, hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304 Flag-mCherry-eEF1A1_IRES-GFP

Plasmid

#198383

Purpose

eEF1A1 fluorescent reporter

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eEF1A1 (EEF1A1 Human)

Use

Lentiviral

Tags

Flag-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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