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pML2(+)WF

Plasmid

#108333

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM860

Plasmid

#84179

Purpose

target miRNA860 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM860

Expression

Plant

Promoter

2x35S

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM1887

Plasmid

#84105

Purpose

target miRNA1887 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM1887

Expression

Plant

Promoter

2x35S

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pFGC5941-PacI-ath-STTM447

Plasmid

#84138

Purpose

target miRNA447 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM447

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM163

Plasmid

#84089

Purpose

target miRNA163 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM163

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProGL2 GW

Plasmid

#80689

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Weinl et al Plant Cell. 2005 Jun;17(6):1704-22. Epub

Type

Empty backbone

Expression

Plant

Promoter

ProGL2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTW322

Plasmid

#115953

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 With 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW323

Plasmid

#115954

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P1 No 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW325

Plasmid

#115956

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 With 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW326

Plasmid

#115957

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 No 3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW327

Plasmid

#115958

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P2 End

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW328

Plasmid

#115959

Purpose

Destination Vector

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl1 P3 With E1-3'UTR

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-LGK/csTEM1

Plasmid

#81164

Purpose

pSALECT plasmid with levoglucosan kinase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase

Tags

Codon swapped TEM-1 beta-lactamase (H26 to W290) …

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pACCS3-1

Plasmid

#78559

Purpose

4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.

Depositor

James Kaper

Article

Giron et al Vaccine. 1995 Jul;13(10):939-46.

Insert

cs3 operon

Expression

Bacterial

Promoter

tet

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-pnr

Plasmid

#71503

Purpose

flySTARR-seq screening vector with pnr core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM390

Plasmid

#84202

Purpose

Disturb the expression of miR390 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR390

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM393

Plasmid

#84203

Purpose

Disturb the expression of miR393 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR393

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM156

Plasmid

#84194

Purpose

Disturb the expression of miR156 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR156

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
pSTAP-seq_fly-ctrl

Plasmid

#86380

Purpose

Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM172

Plasmid

#84100

Purpose

target miRNA172 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM172

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM861-3p

Plasmid

#84180

Purpose

target miRNA861 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM861-3p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSTARR-seq_fly-NipB

Plasmid

#71502

Purpose

flySTARR-seq screening vector with NipB core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB412

Plasmid

#68677

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM319/159

Plasmid

#84116

Purpose

target miRNA319/159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM319/159

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM171

Plasmid

#84098

Purpose

target miRNA171 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM171

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB232

Plasmid

#68623

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM771

Plasmid

#84140

Purpose

target miRNA771 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM771

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB432

Plasmid

#68697

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-EsaR-I70V/D91G

Plasmid

#47644

Purpose

pAC-EsaR with a208g and a272g mutations in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V/D91G

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine and Aspartic acid…

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCRISPRa-Theo-1

Plasmid

#240207

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpRYa-Theo-1

Plasmid

#240208

Purpose

Bacterial SpRYa vector expressing dSpRY, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dSpRY
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRa-Trp

Plasmid

#240209

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a tryptophan-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Tryptophan MR-scRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DoS1Δinc

Plasmid

#244464

Purpose

DoS1 derived plasmid without RP4 inc

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

Plasmid

#235119

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-SlmiR482bTS-B/c

Plasmid

#234368

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK883

Plasmid

#242919

Purpose

Constitutive expression of dCas9, MCP-SoxS-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS-SYNZIP6

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK157

Plasmid

#242908

Purpose

Constitutive expression of dCas9, MCP-SYNZIP6, and αNTD-SYNZIP5

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Inserts

MCP-SYNZIP6
αNTD-SYNZIP5

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK147.02

Plasmid

#242909

Purpose

Constitutive expression of dCas9, PspF-λN22, and boxB scRNA J102

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA boxB J102

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK692

Plasmid

#242910

Purpose

Constitutive expression of dCas9 and αNTD-MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

αNTD-MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK321

Plasmid

#242912

Purpose

aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DoS2-pCU1

Plasmid

#244470

Purpose

Targeting pCU1 plasmid

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

I-SceI enzyme (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DoS1ΔoriT

Plasmid

#244465

Purpose

DoS1 derived plasmid without RP4 oriT

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 13,975 results for: Lor