We narrowed to 18,890 results for: Tec

1. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-RAB-ICUE

   Plasmid

   #118480

   Purpose

   ddRFP-based single-color biosensor for monitoring cAMP dynamics.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-ICUE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. JD631

   Plasmid

   #160399

   Purpose

   Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   35S::vitis GRF4-GIF1 chimera
   

   Use

   Binary vector

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC151

   Plasmid

   #118899

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   GST tag, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9 nickase for Split-PE (pJUL2497)

   Plasmid

   #190104

   Purpose

   Expresses nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Tags

   XTEN-bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-GRAB_ATP1.0mut

   Plasmid

   #167578

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

   Plasmid

   #171438

   Purpose

   Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-c-Jun
   

   Expression

   Mammalian

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB1-spect-PthrC3_8-eGFP

   Plasmid

   #107411

   Purpose

   This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3_8

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTF-lacZ

    Plasmid

    #153036

    Purpose

    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC18/promEco/Lysostaphin

    Plasmid

    #125765

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK-Sa1 promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Promoter

    wheat U6

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. CTB

    Plasmid

    #141166

    Purpose

    Expresses recombinant Cholera Toxin B Subunit

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    CTB
    

    Expression

    Bacterial

    Mutation

    No secretion signal

    Promoter

    T7

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHSB04X

    Plasmid

    #117258

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
    

    Use

    Other

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191182

    Purpose

    Expression of EACTB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:ACTB.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-AFID-3

    Plasmid

    #167361

    Purpose

    For inducing predictable deletions in rice plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 CYP3A4

    Plasmid

    #67104

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP3A4 (CYP3A4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCpf1b

    Plasmid

    #122187

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-mSA*

    Plasmid

    #182952

    Purpose

    Express SpCas9-mSA*

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    SpCas9-mSA*
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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