We narrowed to 18,681 results for: tec

1. ND4.2-Left TALE-G1397-N-wildtype

   Plasmid

   #179684

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM119

   Plasmid

   #127213

   Purpose

   BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   GFP, WUS2, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT451

   Plasmid

   #139391

   Purpose

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BA inducible promoters

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9 Null

   Plasmid

   #198727

   Purpose

   Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ND4.2-Right DdCBE-G1397-C-wildtype

   Plasmid

   #179687

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Cas9-NRCH

   Plasmid

   #136926

   Purpose

   Expresses Cas9-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRCH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF-CjPglB-LpxE

   Plasmid

   #128389

   Purpose

   Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Inserts

   PglB oligosaccharylktransferase
   LpxE phosphatase
   

   Use

   Tags

   Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and pBAD

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ca-FLARE (TF)

    Plasmid

    #92213

    Purpose

    Transcription factor component of FLARE

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCARS1

    Plasmid

    #174868

    Purpose

    Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

    Depositor

    Zengyi Shao

    Article

    Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

    Insert

    ARS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPU-huTRIMmiR30-TRIM5

    Plasmid

    #132938

    Purpose

    All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTPH412

    Plasmid

    #185724

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-gp41-8N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    ecTadA(8e) R26G

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT137-splitC

    Plasmid

    #122605

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

    Plasmid

    #191140

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

    Plasmid

    #194210

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and CJ8421_04975 Rptr2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

    Plasmid

    #191170

    Purpose

    EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (plant)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge