We narrowed to 14,145 results for: TIM
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Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianPromoterUBC humanAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-dCas9
Plasmid#220193Purposemini-Tn7 dCas9 expression vectorDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterpromoterlessAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Strep IIExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH2B-SCFP1-2A-mTq2-NES
Plasmid#60491PurposeCo-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasmDepositorInsertsH2B-SCFP1
mTurquoise2-NES
TagsSCFP1 and mTurquoise2ExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
141_pT3TS_iCre
Plasmid#171793PurposePlasmid for optimised iCre RNA synthesis using T3 promoter.DepositorInsertoptimised Cre for RNA expression
ExpressionBacterialPromoterT3Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH-MoHyPer
Plasmid#78267PurposeExpress HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.DepositorInsertMoHyper
UseExpress in magnaportheExpressionYeastPromoterRp27Available SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B3.TM0851
Plasmid#11530DepositorInsertheat-inducible transcriptional repressor (TM0851 T. maritima)
TagsHisTag, TEV cleavableExpressionBacterialAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRE-pfv-Sapphire
Plasmid#231957PurposeThe plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)DepositorInsertgenetically encoded multimeric nanoparticles
UseLentiviral; Doxocycline inducibleExpressionMammalianPromotertight TRE promoterAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRS-XylS-dCas9
Plasmid#220196Purposemini-Tn7 dCas9 expression vector - XylS/pM promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterXylS/pMAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-dCas9
Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPD_Pinx-1::ChR2(H134R)::mCherry
Plasmid#117420PurposeFor selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
ExpressionWormMutationH134RPromoterinx-1Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
Plasmid#60492PurposeCo-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasmDepositorInserts3xnls-SGFP2(T65G)
SGFP2-3xNES
TagsSGFP2 and SGFP2(T65G)ExpressionMammalianPromoterCMVAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD_Psra-6::ChR2(H134R)::mCherry
Plasmid#117419PurposeFor selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoterDepositorInsertchannel rhodopsin-2 with H134R mutation
ExpressionWormMutationH134RPromotersra-6Available SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-RapACR-EYFP
Plasmid#111075PurposeFast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spikingDepositorInsertAnion channelrhodopsin RapACR
UseLentiviralTagsEYFPAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRS-AraC-dCas9
Plasmid#220195Purposemini-Tn7 dCas9 expression vector - AraC/pBAD promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterAraC/pBADAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterRNR1prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTags6xHis, PelB, and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRibo-APEX2m
Plasmid#176842PurposeExpression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1661-sgMUC4-E3(F+E)
Plasmid#51025PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3DepositorInsertoptimized sgRNA (MUC4 Mouse, Human)
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ heiCas9
Plasmid#186929PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLSDepositorInsertmyc-flexible linker-oNLS-Cas9-oNLS
UseCRISPRTagsmyc-flexible linker-optimizedNLS and optimizedNLSExpressionMammalianPromotersp6Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
TagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only