We narrowed to 8,828 results for: Pol

1. attB-mCh-Dnmt3b1-Poly(A)-NeoR

   Plasmid

   #65553

   Purpose

   Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   isoform 1

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8xCTS1_delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124545

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124544

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41 C55A, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124540

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124538

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast POLD1 ISCmut

   Plasmid

   #160806

   Purpose

   Express ISC mutant POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 ISCmut (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids, C1058…

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. attB-GFP-Tet1d834-1363-Poly(A)

   Plasmid

   #65560

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   del(834-1363)

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. attB-GFP-Dnmt3b1-Poly(A)-NeoR

   Plasmid

   #65551

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 1

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. KY801: pMVP (L3-L2) P2A-eGFP + polyA

   Plasmid

   #121764

   Purpose

   pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-eGFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. attB-GFP-Tet1d834-1053-Poly(A)

    Plasmid

    #65561

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    del(834-1053)

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp211-P30-His-pol30(K127R)-3'

    Plasmid

    #99547

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1203R-CMV-3'dmDR-EGFP-bGHpolyA

    Plasmid

    #220962

    Purpose

    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1203D-5'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221534

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1203E-3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221535

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221536

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1203O-CMV-DiCas7-11-Δ7.1-bGHpolyA

    Plasmid

    #220959

    Purpose

    DiCas7-11 effector with Cas7.1 domain deletion mutation

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid

    #220961

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Poliovirus 1 PV1 isolate 09_05_19 3C protease

    Plasmid

    #228647

    Purpose

    Bacterial expression of the Polio 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Polio virus 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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