We narrowed to 13,926 results for: RING

1. NAX_CAGGS_CAS9_m4_EYFP

   Plasmid

   #167866

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Geph UTR shRNA

   Plasmid

   #121070

   Purpose

   shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

   Depositor

   Angel de Blas

   Article

   Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

   Insert

   GPHN shRNA (Gphn Rat)
   

   Use

   RNAi

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTL472

   Plasmid

   #69882

   Purpose

   dCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassette

   Depositor

   Robert Kittel, Tobias Langenhan

   Article

   Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

   Insert

   CIRL (Cirl Fly)
   

   Use

   Transformation vector

   Tags

   FLAG

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROP_C9_Puro_HDAC3

   Plasmid

   #183299

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   HDAC3
   

   Use

   Lentiviral

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PMV-ycrtE

   Plasmid

   #65341

   Purpose

   systhesis of crtE gene in the beta-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   ycrtE
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROP_C9_Puro_ESR1

   Plasmid

   #183288

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   ESR1
   

   Use

   Lentiviral

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL SFFV Sbf-Sal linker

   Plasmid

   #50434

   Purpose

   Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

   Depositor

   Andrew Scharenberg

   Article

   Boissel et al Nucleic Acids Res. 2013 Nov 26.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-Skp-hLYZ

   Plasmid

   #83924

   Purpose

   Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   lysozyme (LYZ Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC020.306

   Plasmid

   #171148

   Purpose

   Expression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSuper.Retro.Puro-PKN1 shRNA 4

    Plasmid

    #59978

    Purpose

    Knockdown of PKN1

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    PKN1 shRNA
    

    Use

    Retroviral

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

11. S3_002_026_096

    Plasmid

    #134403

    Purpose

    pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Tags

    None and none

    Expression

    Yeast

    Mutation

    None and none

    Promoter

    pGPM1 and pRPL18B

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. FAK

    Plasmid

    #186141

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_A0301

    Plasmid

    #91005

    Purpose

    Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A nickase
    

    Use

    CRISPR

    Mutation

    H840A

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. BC-A1-005

    Plasmid

    #78692

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBpuro-LDR

    Plasmid

    #173857

    Purpose

    Encoding a part of the rapamycin-induced dimerization system.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    a Lyn11-targeted FRB (LDR)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0.017

    Plasmid

    #119621

    Purpose

    Level 0 Part. CDS

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Mutation

    bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pES002 (pTet-qacR wt)

    Plasmid

    #69031

    Purpose

    wild-type qacR downstream of a Tet promoter

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Insert

    qacR (qacR Synthetic)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB1A2QS

    Plasmid

    #138268

    Purpose

    MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity

    Depositor

    Xiao Wang

    Article

    Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Inserts

    AraC-TetR operon
    LuxR-LacI operon
    mCherry
    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Para/lac and Plux/tet

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK110

    Plasmid

    #99535

    Purpose

    contains bgl operon sequence for E. coli in vitro transcription template

    Depositor

    Robert Landick

    Article

    Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

    Insert

    E. coli bgl antisense region containing bglG
    

    Tags

    None

    Promoter

    lambda Pr promoter used for in vitro transcriptio…

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMP315

    Plasmid

    #134994

    Purpose

    KRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pUBC-KRAB-NLS-DpnI(aa146-254)
    

    Tags

    DpnI(aa146-254) and KRAB domain

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDD2703

    Plasmid

    #175079

    Purpose

    Mammalian expression of Flag-tagged KSHV ORF45 E223A-S226A mutant

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

    Insert

    KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8 )
    

    Expression

    Mammalian

    Mutation

    E223A, S226A

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAP1837

    Plasmid

    #137086

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAP3041

    Plasmid

    #137083

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    GFP11::3xFlag::tagRFP
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101115

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII

    Plasmid

    #80909

    Purpose

    Phagemid (encodes cI4A5C6G7G and M13 gene III)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    cI4A5C6G7G
    

    Expression

    Bacterial

    Mutation

    238G to 238D (please see depositor comment below)

    Promoter

    BBa_J23106 and rpoN

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Promoter

    T7

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLKO.1 EB1 shRNA #1

    Plasmid

    #37928

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 shRNA #1 (MAPRE1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. pYPQ141-ZmUbi-RZ-Aa3.8.4

    Plasmid

    #136375

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCfB6681

    Plasmid

    #106157

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMOD_A3910

    Plasmid

    #91054

    Purpose

    Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    dTaCas9-VPR
    

    Use

    CRISPR

    Promoter

    ZmUbi

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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