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Showing: 11451 - 11500 of 75864 results

  1. pGustiv

    Plasmid
    #240335
    Purpose
    Expresses BK polyomavirus genotype IV capsid protein VP1 in yeast. Expression of VP1 (and a GFP reporter) is induced by maltose.
    Depositor
    Christopher Buck
    Inserts
    BKV-IV VP1
    Superfold GFP
    Formaldehyde dehydrogenase 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    FDH1, MAL31, and MAL32
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    240335-plasmid-map-sequence-id-478372

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  2. pAG CMV HA-EGFP Puro

    Plasmid
    #237882
    Purpose
    Mammalian HA-EGFP expression plasmid, control plasmid for Addgene #237879
    Depositor
    Addgene Research Program, Meghan Rego
    Insert
    EGFP
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237882-plasmid-map-sequence-id-471570

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  3. pAG CMV EGFP-HA Puro

    Plasmid
    #237883
    Purpose
    Mammalian EGFP-HA expression plasmid, control plasmid for Addgene #237880
    Depositor
    Addgene Research Program, Meghan Rego
    Insert
    EGFP
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237883-plasmid-map-sequence-id-469908

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  4. pAG CMV EGFP Puro

    Plasmid
    #237884
    Purpose
    Mammalian EGFP expression plasmid, control plasmid for Addgene #237881
    Depositor
    Addgene Research Program, Meghan Rego
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237884-plasmid-map-sequence-id-469914

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  5. Ec FkP-dnaN-pRSFDuet

    Plasmid
    #238342
    Purpose
    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta(wt) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (wt) (dnaN E. coli)
    Use
    Tags
    3X FLAG+PKA kinase motif+PreScission Protease cle…
    Expression
    Bacterial
    Mutation
    none (wt)
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238342-plasmid-map-sequence-id-472702

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  6. Untagged TDP-43

    Plasmid
    #237551
    Purpose
    Expression of untagged wild-type TDP-43
    Depositor
    Tatyana Shelkovnikova
    Insert
    TDP-43 WT (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild-type TDP-43
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    237551-plasmid-map-sequence-id-471601

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  7. pML107-NET1

    Plasmid
    #232893
    Purpose
    Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    NET1 gRNA (NET1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232893-plasmid-map-sequence-id-470393

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  8. pML107-VRG4

    Plasmid
    #232899
    Purpose
    Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VRG4 gRNA (VRG4 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232899-plasmid-map-sequence-id-471178

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  9. pML107-SAM50

    Plasmid
    #232895
    Purpose
    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SAM50 gRNA (SAM50 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232895-plasmid-map-sequence-id-470389

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  10. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Use
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

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  13. TRE-FRT-MCS-WPRE-SV40 - GCH27

    Plasmid
    #230828
    Purpose
    AAV cloning vector for Flpo-dependent expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector, flp/frt
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230828-plasmid-map-sequence-id-466645

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  14. pAN68

    Plasmid
    #164525
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    -6xhis-precission-GFP
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    164525-plasmid-map-sequence-id-328004

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  15. pAG Retro EGFP Neo

    Plasmid
    #234464
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234464-plasmid-map-sequence-id-464634

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  16. pCS2-zH2B-GFP

    Plasmid
    #226561
    Purpose
    Express H2B-GFP in zebrafish.
    Depositor
    Yong Zhang
    Insert
    h2b (h2bc1 Zebrafish)
    Use
    Synthetic Biology; Zebrafish expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    226561-plasmid-map-sequence-id-461800

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  17. pGL3_DDX25

    Plasmid
    #232359
    Purpose
    Enhancer near DDX25, chr11:125935511-125936012 cloned into pGL3
    Depositor
    Joanna Wysocka
    Insert
    DDX25 (DDX25 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232359-plasmid-map-sequence-id-463060

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  18. pGL3_MSR1_rightneg0topos1

    Plasmid
    #232389
    Purpose
    MSR1 enhancer, right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, right buffering Coordinator to sen…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232389-plasmid-map-sequence-id-462852

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  19. pML104-HygMx4-URA3-3

    Plasmid
    #232886
    Purpose
    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232886-plasmid-map-sequence-id-462214

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  20. pML104-HygMx4-URA3-2

    Plasmid
    #232885
    Purpose
    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    URA3 gRNA (URA3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232885-plasmid-map-sequence-id-462211

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  21. MigR1 GFP Mouse PHGDH

    Plasmid
    #220591
    Purpose
    Retroviral mammalian expression vector with GFP for overexpression of mouse PHGDH
    Depositor
    Russell Jones
    Insert
    Ms PHGDH (Phgdh Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    220591-plasmid-map-sequence-id-436554

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  22. pML104-HIS3-3

    Plasmid
    #232882
    Purpose
    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232882-plasmid-map-sequence-id-462144

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  23. pML107-ZIM17

    Plasmid
    #232901
    Purpose
    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ZIM17 gRNA (ZIM17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232901-plasmid-map-sequence-id-462145

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  24. pML104-HIS3-2

    Plasmid
    #232881
    Purpose
    Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232881-plasmid-map-sequence-id-462138

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  25. pML107-ADE13

    Plasmid
    #232887
    Purpose
    Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ADE13 gRNA (ADE13 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232887-plasmid-map-sequence-id-462137

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  26. pML107-LAS17

    Plasmid
    #232892
    Purpose
    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LAS17 gRNA (LAS17 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232892-plasmid-map-sequence-id-462139

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  27. pMX-Flag-GFP_EDIL3

    Plasmid
    #227957
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    EDIL3 (EDIL3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    227957-plasmid-map-sequence-id-461332

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  28. AAV-hSyn-PiGM-Iq(535/81, eGFP)

    Plasmid
    #221612
    Purpose
    AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Iq (535/81,eGFP)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS2 1-53 truncation
    Promoter
    Human Synapsin
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221612-plasmid-map-sequence-id-461237

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  29. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

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  30. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

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  31. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

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  32. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

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  33. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

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  37. pMX-Flag-GFP_TMEM256

    Plasmid
    #227955
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    TMEM256 (C17orf61 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227955-plasmid-map-sequence-id-455415

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  38. pMX-Flag-GFP_CBLN3

    Plasmid
    #227950
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    CBLN3 (CBLN3 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227950-plasmid-map-sequence-id-455399

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  40. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

    Plasmid
    #227089
    Purpose
    Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)
    Depositor
    Chris Hill
    Insert
    tom1p-HECT (TOM1 Budding Yeast)
    Use
    Tags
    HIS-MBP-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227089-plasmid-map-sequence-id-451122

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  41. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

    Plasmid
    #227085
    Purpose
    Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227085-plasmid-map-sequence-id-451055

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  42. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

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  43. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  44. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid
    #217323
    Purpose
    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217323-plasmid-map-sequence-id-438442

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  45. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid
    #217320
    Purpose
    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217320-plasmid-map-sequence-id-438445

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  46. AAV-GFAPp-PiGM-Iq(mCherry)

    Plasmid
    #221609
    Purpose
    A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and mCherry as expression marker. GFAP promoter for astrocyte-selective expression.
    Depositor
    John Lin
    Insert
    PiGM-Iq (D387A, mCherry)
    Use
    AAV
    Tags
    Expression
    Mutation
    RGS2 1-53 truncation, CRYPHR D387A
    Promoter
    GFAP promoter
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    221609-plasmid-map-sequence-id-477209

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  47. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

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  48. GBH-2aG4-insBFP.1

    Plasmid
    #188025
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188025-plasmid-map-sequence-id-381128

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  49. GBH-2aG4-insGFP.1

    Plasmid
    #188028
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188028-plasmid-map-sequence-id-381113

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  50. GBH-2aG4-insGFP.2

    Plasmid
    #188029
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188029-plasmid-map-sequence-id-381142

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