We narrowed to 22,807 results for: crispr

1. pBzCas13b-gRNA-3

   Plasmid

   #164860

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. OA-1050R (Ubiq-dCasRx)

   Plasmid

   #132417

   Purpose

   Express dCasRx under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Insert

   dCasRx
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ubiq

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. OA-1050L (UASt-CasRx)

   Plasmid

   #132418

   Purpose

   Express CasRx under UASt promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

   Inserts

   CasRx
   UASt+hsp70
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UASt

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSM-dCas9::Ap

   Plasmid

   #154307

   Purpose

   Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

   Plasmid

   #138312

   Purpose

   Lenti expression of AcrIIA14, C-terminus

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA14, C terminus
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Himar-dCas9-mammalian

   Plasmid

   #138414

   Purpose

   Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   3xFLAG, 6xHis, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFETCh_ZHX3_iso1

   Plasmid

   #128239

   Purpose

   Donor vector for 3' FLAG tag of human ZHX3_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZHX3_iso1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFETCh_SSX2B

   Plasmid

   #128241

   Purpose

   Donor vector for 3' FLAG tag of human SSX2B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   SSX2B Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFETCh_ZNF326_iso1

   Plasmid

   #128236

   Purpose

   Donor vector for 3' FLAG tag of human ZNF326_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZNF326_iso1 Homology arms
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_ZBTB49_iso1

    Plasmid

    #128240

    Purpose

    Donor vector for 3' FLAG tag of human ZBTB49_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB49_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAZ.1.0-gDNA

    Plasmid

    #112455

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ

    Depositor

    Kevin White

    Insert

    MAZ (MAZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHRB.1.0-gDNA

    Plasmid

    #112429

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB

    Depositor

    Kevin White

    Insert

    THRB (THRB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. dSp-Rta 100-175

    Plasmid

    #99667

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain (101-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dSp-Rta 50-175

    Plasmid

    #99665

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain (51-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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