Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 9201 - 9240 of 18759 results

  2. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid
    #135014
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the C-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135014-plasmid-map-sequence-id-267599

    Enlarge

  3. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid
    #135012
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3x Flag, 3xFLAG (N terminal on insert), NLS, and …
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135012-plasmid-map-sequence-id-267596

    Enlarge

  11. GWF180

    Plasmid
    #133607
    Purpose
    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9
    Depositor
    Dustin Maly
    Publication
    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    Expression
    Mammalian
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    133607-plasmid-map-sequence-id-261658

    Enlarge

  15. pcDNA-Flag-LmoCas8b2

    Plasmid
    #126482
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas8b2
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126482-plasmid-map-sequence-id-246628

    Enlarge

  16. pcDNA-Flag-LmoCas7

    Plasmid
    #126483
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas7
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126483-plasmid-map-sequence-id-246629

    Enlarge

  17. pcDNA-Flag-LmoCas5

    Plasmid
    #126484
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas5
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126484-plasmid-map-sequence-id-246630

    Enlarge

  20. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid
    #129412
    Purpose
    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgACSL3.C (ACSL3 Human)
    Use
    CRISPR
    Tags
    P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129412-plasmid-map-sequence-id-254823

    Enlarge

  25. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid
    #126425
    Purpose
    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    alanine-6 of TdT has been changed to a STOP codon
    Promoter
    pCMV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126425-plasmid-map-sequence-id-252600

    Enlarge

  31. pTBL210

    Plasmid
    #126451
    Purpose
    Expresses Cas9-XTEN-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126451-plasmid-map-sequence-id-245970

    Enlarge

Showing: 9201 - 9240 of 18759 results