We narrowed to 24,626 results for: Nes

1. pN1-RnKIF5C(1-560)-Halo-Flag

   Plasmid

   #188071

   Purpose

   Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

   Depositor

   Kristen Verhey

   Article

   Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

   Insert

   rat KIF5C (Kif5c Rat)
   

   Use

   Tags

   Flag and Halo

   Expression

   Mammalian

   Mutation

   aa1-560

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-YAP1 CA

   Plasmid

   #79502

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3XFLAG

   Expression

   Mutation

   S127A

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-Gaolf-Integration

   Plasmid

   #129456

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   G alpha olf (Gnal Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-ddMSK1

   Plasmid

   #165603

   Purpose

   Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, S. Pyogenes, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

   Plasmid

   #114103

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

   Insert

   sbGLuc-VChR1-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   motoneuron-specific Homeobox 9 promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-EF1.Pro

   Plasmid

   #79488

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   EF1 Promoter (EEF1A1 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   EF1 Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-IL6.Pro

   Plasmid

   #79491

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Il6 Promoter (IL6 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   IL6 Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. G-GECO1-Orai1

   Plasmid

   #73561

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   NM_032790.3 (ORAI1 Human, Synthetic)
   

   Use

   Tags

   G-GECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC-BAR-YR

    Plasmid

    #61766

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-CAD

    Plasmid

    #73566

    Purpose

    Soluble red fluorescent activator of Orai1 channels

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    mCherry-CAD (STIM1 342-448) (STIM1 Human, Synthetic)
    

    Use

    Tags

    6x His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid

    #184562

    Purpose

    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines.

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid

    #149277

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-4eCOL2A1

    Plasmid

    #97211

    Purpose

    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Vector is designed for cloning and generation of …

    Tags

    Expression

    Mutation

    Promoter

    COL2A1 regulatory elements

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid

    #68218

    Purpose

    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc / Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos / 35S / 35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TKTL1

    Plasmid

    #72419

    Purpose

    Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis

    Depositor

    David Sidransky

    Article

    Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66

    Insert

    TKTL1 (TKTL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    may have one or two synonymous substitutions

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pEGFP-Sec22b-shR

    Plasmid

    #208358

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations introduced to render shRNA …

    Promoter

    CMV

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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