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pCMV-Tag2B-E2F1

Plasmid

#202520

Purpose

Expressing human E2F1 protein with FLAG-tag

Depositor

Ming Chen

Publication

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

E2F1 (E2F1 Human)

Tags

FLAG-tag

Expression

Mammalian

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCyCEN_Lisp2mCherry_hsp70_GFP

Plasmid

#137169

Purpose

Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

Depositor

Clemens Kocken

Publication

Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

Inserts

mCherry
Nanoluc
dihydrofolate reductase
Green Fluorescent Protein

Use

Unspecified

Tags

T2A

Promoter

P. cynomolgi hsp70 and P. cynomolgi lisp2

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pE-SUMO CRYBA3

Plasmid

#80747

Purpose

Expression of human beta A3-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA3 (CRYBA1 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCY 3080-07

Plasmid

#36227

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-Zeocin

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xHA-IRES-blasticidin

Plasmid

#255989

Purpose

PMXS construct expressing EMC3-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xHA (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xHA

Expression

Mammalian

Available since

June 12, 2026

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-b3

Plasmid

#189797

Purpose

Expression of full length human integrin beta 3

Depositor

Timothy Springer

Publication

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

integrin b3 (ITGB3 Human)

Tags

-P2A-mCherry

Expression

Mammalian

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 4NQ

Plasmid

#121456

Purpose

expression of human PD-L1 mutant in mammalian cells

Depositor

Mien-Chie Hung

Publication

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAD104_Asn152Ser_pCMV6Entry-hIRG1

Plasmid

#124877

Purpose

Expresses hCAD Asp152Ser mutant in mammalian cells

Depositor

Konrad Buessow

Publication

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Asn152 to Ser

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-DAP5-3XFLAG

Plasmid

#127330

Purpose

Express 3xFLAG tagged DAP5 protein from GUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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LC001: pMVP/PB/Hygro-DEST

Plasmid

#121876

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-Gamma-Tubulin-17

Plasmid

#57721

Purpose

Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mOrange2-LaminB1-10

Plasmid

#57966

Purpose

Localization: Nuclear Envelope, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LaminB1 (LMNB1 Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag-YAP-S381A

Plasmid

#33068

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2+ Bmi1-cherry-HA

Plasmid

#30516

Depositor

John Gurdon

Publication

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bmi1 (Bmi1 Mouse)

Use

Xenopus/zebrafish expression

Tags

cherry and triple HA

Expression

Mammalian

Mutation

NO STOP

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Publication

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCY 3010-07

Plasmid

#36212

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-Zeocin

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA7woH

Plasmid

#65020

Purpose

Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, E105K, E108A, D16…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PRMT2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176953

Purpose

Mammalian expression vector encoding PRMT2 and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

PRMT2 (PRMT2 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Publication

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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