We narrowed to 24,626 results for: Nes

1. pAAV-FLEXfrt-SaCas9-U6-sgGria1

   Plasmid

   #124867

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-FLEXfrt-SaCas9-U6-sgGria2

   Plasmid

   #124868

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe JunD-HA neo

   Plasmid

   #58486

   Purpose

   mammalian expression of mouse JunD

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JunD (Jund Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXPE

   Plasmid

   #15284

   Depositor

   Ron Vale

   Article

   Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

   Insert

   ubiquitous kinesin heavy chain (KIF5B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The insert is missing the 1st 180 bp of 5' u…

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. CMV-CAD

   Plasmid

   #73567

   Purpose

   Soluble activator of Orai1 channels

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   CAD (STIM1 342-448) (STIM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Plasmid

   #101211

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Plasmid

   #101204

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

   Plasmid

   #149278

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Promoter

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187945

    Purpose

    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    Synthetic Biology; Piggybac

    Tags

    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-FLEX-SaCas9-U6-sgGria3

    Plasmid

    #124855

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEN_TTmiRc2_3xflag_NRF2

    Plasmid

    #136527

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

    Plasmid

    #124865

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX302 ID2-V5 puro

    Plasmid

    #83096

    Purpose

    Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    ID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRF.1 udsVenus (P_JUND)

    Plasmid

    #58692

    Purpose

    A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    endogenous promoter of JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    udsVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

17. TET-pLKO.1 PURO shID2 #2

    Plasmid

    #83087

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human ID2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shID2 (ID2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pEN_TT 3xFLAG-MAVS

    Plasmid

    #158628

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-TRE-DIO-tdTomato-f

    Plasmid

    #127092

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tdTomato-f
    

    Use

    AAV

    Tags

    farnesylation signal from c-Ha-Ras

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK_hygro_BirA_CDKN1A

    Plasmid

    #136538

    Purpose

    Lentiviral expression vector for an inducible BirA-CDKN1A

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA-CDKN1A (CDKN1A )
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge