We narrowed to 5,834 results for: SPI

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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CRY-tTA (B702)

Plasmid

#92032

Purpose

Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

tTA2 (TetR fused to VP64)

Expression

Mammalian

Mutation

NLS on CRY2 is mutated

Promoter

CMV

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL WT 3xFlag-V5

Plasmid

#174129

Purpose

Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_81-236-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH-SWAP70(R223E, R224E)

Plasmid

#84583

Purpose

Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306, mut…

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S708/710A

Plasmid

#70153

Purpose

mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S708A, S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp4-a del.SHD

Plasmid

#40067

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

deleted SHD domain (1-143)

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

Plasmid

#39859

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S708A

Plasmid

#70146

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S708A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-ETWV

Plasmid

#133034

Purpose

Expresses Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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5' MBD3 TALEN

Plasmid

#52358

Purpose

TALEN vector for targeting human MBD3 locus (5')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NI NN NN NN NN HD HD NI NN NG NG NN NG NN NN NN NN NG NN

Use

TALEN

Promoter

CAGGS

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
SNAIL-Luc

Plasmid

#250936

Purpose

Lentiviral vector encoding a truncated promoter region of human SNAIL gene driving a luciferase reporter

Depositor

Michelle Farkas

Article

Chhe et al Clocks Sleep. 2025 Sep 28;7(4):54. doi:

Insert

SNAIL promoter (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Contains a 340 bp region of the truncated SNAIL p…

Promoter

SNAIL

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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bRA66-Mic60chimeras

Plasmid

#245525

Purpose

Edited bRA66 containing gRNA to create S. cerevisiae Mic60 chimeras

Depositor

Luke Chao

Article

Benning et al Protein Sci. 2025 Jul;34(7):e70207. doi:

Insert

YKR016w (MIC60 Budding Yeast)

Use

CRISPR

Promoter

T7 TAATACGACTCACTATAGG

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-EGFR-FCRL3-140aa-Chimera-puro

Plasmid

#248151

Purpose

Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its full-length cytoplasmic tail, fused to the extracellular domain of EGFR.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

EGFR-FCRL3 (FCRL3 Human)

Use

Lentiviral

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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