164,577 results

1. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT-R2Tg-WT

   Plasmid

   #238105

   Purpose

   For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   Wildtype R2Tg, mammalian codon optimized
   

   Use

   For ivt

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Flag Pu.1

   Plasmid

   #66974

   Purpose

   Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Thy1.1-G5

   Plasmid

   #234839

   Purpose

   pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

   Depositor

   Gabriel Victora

   Article

   Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

   Insert

   Thy1.1 (Thy1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-P2A-5xGly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)Flag-His-ATM wt

   Plasmid

   #31985

   Purpose

   Mammalian expression of human ATM with Flag and His tags

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM wild-type (ATM Human)
   

   Use

   Tags

   Flag and HIS

   Expression

   Mammalian

   Mutation

   3' UTR of 400 nucl.

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+DPP6

   Plasmid

   #117272

   Purpose

   Encodes human DPP6 variant 1

   Depositor

   Mark Cannell

   Article

   Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

   Insert

   DPP6 (DPP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVTNT-T7-KPNA5

   Plasmid

   #26681

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 5 (KPNA5 Human)
   

   Use

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/Flag-WDR5

    Plasmid

    #29759

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid

    #148120

    Purpose

    Mammalian Expression of HshnRNPH1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPH1 (HNRNPH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVTNT-T7-KPNA6

    Plasmid

    #26682

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 6 (KPNA6 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Pur-CAG-hM3Dq-mCherry

    Plasmid

    #80948

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM3Dq-mCherry
    

    Use

    Tags

    mcherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGAMS-mEos2

    Plasmid

    #105128

    Purpose

    Expresses human FGAMS-mEos2 fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    French et al Science. 2016 Feb 12;351(6274):733-7. do

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-Gsdmd-NT

    Plasmid

    #80951

    Purpose

    Expresses Gsdmd-NT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. dimericTomato-2xrGBD

    Plasmid

    #176098

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMVdel

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    LifeAct
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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