We narrowed to 23,617 results for: CHI

1. pMXLC_pFa

   Plasmid

   #114219

   Purpose

   POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

   Depositor

   Christopher A. O'Callaghan

   Article

   Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

   Insert

   LacZalpha
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW8ENdeI-spo5DSR

   Plasmid

   #48983

   Purpose

   for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV0586

   Plasmid

   #133473

   Purpose

   Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOGG050

   Plasmid

   #113999

   Purpose

   SC module. Thermostable beta-galactosidase reporter

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   celB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGG083

   Plasmid

   #114001

   Purpose

   SC module. Beta-glucosidase reporter

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRC246

   Plasmid

   #123038

   Purpose

   Core promoter

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   pLEU2m [2b]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Mouse NHERF-1

   Plasmid

   #32950

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

   Insert

   Slc9a3R1 (Nherf1 Mouse)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   t3

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK220

   Plasmid

   #71123

   Purpose

   Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   pantothenate kinase
   

   Use

   Tags

   H6

   Expression

   Bacterial

   Mutation

   Arg175 is mutated to a His

   Promoter

   T7

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST50Trc2-DHFRHIS

   Plasmid

   #63967

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPTK013-3a-Invertase-αMFΔ

    Plasmid

    #84972

    Purpose

    Type 3a, Invertase followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Invertase followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDUET-ctCPR-sAMO

    Plasmid

    #20111

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and codon optimized amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. Reporter

    Plasmid

    #36008

    Depositor

    Larry Gerace

    Article

    Ohba et al J Cell Biol. 2004 Dec 20;167(6):1051-62.

    Insert

    LAP2β-TM
    

    Use

    Reporter

    Tags

    11kD FRB and EGFP

    Expression

    Mammalian

    Mutation

    FRB is residues 2025-2114 of FRAP, LAP2β-TM is re…

    Promoter

    CMV

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pPTK016-3-RFP_Intra

    Plasmid

    #84975

    Purpose

    Type 3, Red fluorescent protein

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    BsaI site removed (1677 c to g and 1794 a to g)

    Promoter

    Non

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUS_A1A

    Plasmid

    #43848

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFETCh_SIX4_iso1

    Plasmid

    #128238

    Purpose

    Donor vector for 3' FLAG tag of human SIX4_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SIX4_iso1 Homology arms
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOGG039

    Plasmid

    #113996

    Purpose

    SC module. Terminator for T7RNAP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Terminator for T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-ClbN-NHis

    Plasmid

    #51497

    Purpose

    NRPS module containing CATE domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbN
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa30-1094 of NP_754347.1

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST50Trc3-CBPHISNDHFR

    Plasmid

    #63981

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Calmodulin Binding Peptide (CBP); TEV…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDUET-ctCPR-trAMO

    Plasmid

    #20112

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and truncated amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. BtuCD

    Plasmid

    #92208

    Purpose

    Expression of BtuCD in E. coli

    Depositor

    Douglas Rees

    Article

    Locher et al Science. 2002 May 10;296(5570):1091-8.

    Inserts

    btuC (btuC )
    btuD (btuD )
    

    Use

    Tags

    10x Histidine Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pWS788

    Plasmid

    #123059

    Purpose

    _-factor protease

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Bar1 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pN23-LIC-His-FLAG

    Plasmid

    #165478

    Purpose

    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    10xHis-FLAG-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSaI20

    Plasmid

    #8615

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminal threnine changed to alanine

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB8818

    Plasmid

    #126909

    Purpose

    Template for PCR amplification of HMG1from Yarrowia lipolytica

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    YlHMG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWS932

    Plasmid

    #123055

    Purpose

    Pheromone responsive domain

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    PRD [3b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWS2119

    Plasmid

    #123048

    Purpose

    PbPeptide GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    PbSte2 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWS033

    Plasmid

    #123056

    Purpose

    Green fluorescent protein

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    sfGFP [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPTK014-3a-Killer-αMFΔ

    Plasmid

    #84973

    Purpose

    Type 3a, Killer followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Killer followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLNL1130

    Plasmid

    #160861

    Purpose

    PJ23110-RBS-AbWS/DGAT-mRuby2-tL3S2P24_KanR_p15A

    Depositor

    John Dueber

    Article

    Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

    Insert

    O-acyltransferase WSD
    

    Use

    Synthetic Biology

    Tags

    mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23110

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pD2-dcas9-J23101-GFP

    Plasmid

    #122260

    Purpose

    Constitutive dCas9 and GFP expression plasmid

    Depositor

    Chueh Loo Poh

    Article

    Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge