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We narrowed to 58,900 results for: tra

Showing: 4661 - 4680 of 58900 results
  1. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    Insert
    citrate synthase
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. {Ud}RpL14.Dm

    Plasmid
    #53220
    Purpose
    {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescue
    Depositor
    Insert
    RpL14 (RpL14 Fly)
    Use
    Attb drosophila melanogaster transformation
    Mutation
    14 synonymous mutations introduced
    Promoter
    endogenous promoter
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. tRECs/3xUAS-FP attB

    Plasmid
    #220330
    Purpose
    encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)
    Depositor
    Inserts
    B3RT-STOP-B3RT-TagBFP-3xHA
    KDRT-STOP-KDRT-GFP-HSV
    FRTm71-STOP-FRTm71-mCherry
    Expression
    Insect
    Promoter
    UAS
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pT2iaTubG4FER3(#940)

    Plasmid
    #184071
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish transient transgenic
    Depositor
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transient transgenesis
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Gfish-a1Tub
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pSG08-pLac-NRII_H139N

    Plasmid
    #102465
    Purpose
    Expresses NRII protein with H139N mutation in E. coli. The expression is controlled by a pLac promoter.
    Depositor
    Insert
    NRII (ntrB )
    Expression
    Bacterial
    Mutation
    Histidine 139 to Asparagine
    Promoter
    pLac
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 4661 - 4680 of 58900 results