We narrowed to 9,310 results for: Coli

1. p202-Syt1-C2A-I248V

   Plasmid

   #201554

   Purpose

   Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Synaptotagmin-1 C2A domain (partial)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Isoleucine 248 to Valine (numbering based…

   Promoter

   RBS

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet28b_His_RAD23A-UBA1

   Plasmid

   #201549

   Purpose

   Expresses UBA1 of human RAD23A in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 160-205 of DNA polymerase i…

   Promoter

   T7

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1BR9-AtWUSSTOP-Δα3

   Plasmid

   #202056

   Purpose

   E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
   

   Use

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Mutation

   third helix of homeodomain deleted (AA82-102)

   Promoter

   T7 Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

   Plasmid

   #182423

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-eythropoietin (EPO Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDR111_mannose_Stat1Klf6

   Plasmid

   #188400

   Purpose

   Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Inserts

   Stat-1 (Stat1 Mouse, Escherichia coli)
   Klf6 (Klf6 Mouse)
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET303-ColE1-TR-E192C-del1-40

   Plasmid

   #180235

   Purpose

   Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   Colicin E1
   

   Use

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Truncation of ColE1 containing T and R domains (r…

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

   Plasmid

   #186414

   Purpose

   AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli)
   

   Use

   Gateway destination vector

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   Basal promoter of the FOG gene

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCB’ CsoS2 ΔNTD

   Plasmid

   #162705

   Purpose

   Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deletion of residues 2-256 of CsoS2, Y131D

   Promoter

   PLtet0-1 promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC002

   Plasmid

   #171139

   Purpose

   For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J1-BBa_J23117-sfGFP
   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   J1-BBa_J23117 and Sp.pCas9, BBa_J23107

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCM’ CbbQ-

    Plasmid

    #162711

    Purpose

    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Inactivation of CbbQ subunit of CbbOQ rubisco act…

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human INPP5A

    Plasmid

    #129587

    Purpose

    Expression of human INPP5A in bacteria

    Depositor

    Christophe Erneux

    Article

    Erneux et al Eur J Biochem. 1995 Dec 1;234(2):598-602

    Insert

    Type I inositol 5-phosphatase (INPP5A Human)
    

    Use

    Tags

    Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZ_P-ICL1-LacI-Mit1AD

    Plasmid

    #126721

    Purpose

    LacI-Mit1AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Mit1 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZ_P-ICL1-LacI-VP16

    Plasmid

    #126723

    Purpose

    LacI-VP16 on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-VP16 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZ_P-ICL1-LacI-Gal4AD

    Plasmid

    #126724

    Purpose

    LacI-Gal4AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Gal4 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pK_P-GAP-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126745

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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