We narrowed to 16,474 results for: por

1. AID-BFP-loxP_myo2_neoR

   Plasmid

   #194054

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   bfp

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-uTEV1-ACBD5

   Plasmid

   #171008

   Purpose

   HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-ACBD5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-TPI_PTC160-xrRNA-4H

   Plasmid

   #108369

   Purpose

   Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PTC160

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBP-4_1.sensor

   Plasmid

   #162844

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   4_1_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBP-4_1m.mutant

   Plasmid

   #162869

   Purpose

   Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   4_1m_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNHT7::RPL15::sNluc2

   Plasmid

   #186763

   Purpose

   An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRD4 CRISPRi plasmid

   Plasmid

   #154890

   Purpose

   Human BRD4 CRISPRi gRNA

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   BRD4-targeting gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX458-sgRNA_Ago2_3

   Plasmid

   #73531

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL7.0-iRFP670-micro

   Plasmid

   #135954

   Purpose

   Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   iLID micro (SspB R73Q)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/hArf4(T31N)-HA

    Plasmid

    #79405

    Purpose

    Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T31N

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pCAG/hArf4(Q71L)-HA

    Plasmid

    #79404

    Purpose

    Expresses C-terminally HA-tagged Arf4(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Gln 71 Leu

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pET24a-LaM4-1xTS

    Plasmid

    #162778

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-sgRNA_Ago2_4

    Plasmid

    #73532

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CFF-EGFP

    Plasmid

    #86061

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains luminal and transmembrane domains of CD8…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX-eSOX17WHC

    Plasmid

    #118839

    Purpose

    Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSOX17WHC (Sox17 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    WHC: L46W, V53H, E57C

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-uTEV1-SQStmd

    Plasmid

    #171009

    Purpose

    HiLITR protease with SQS C-terminal targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs_SFXN1

    Plasmid

    #110635

    Purpose

    Retroviral expression of SFXN1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-h-mmPtch1-B-HA-GFP

    Plasmid

    #120903

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-GSDMD_C192A-Myc

    Plasmid

    #201168

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    C192A

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG/hArf4(WT)-HA

    Plasmid

    #79403

    Purpose

    Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pEN_TT 3xFLAG-KPNA1

    Plasmid

    #192304

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA1 (KPNA1 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAD-CMV-STAT5A-DN-CMV-GFP

    Plasmid

    #83252

    Purpose

    Adenoviral expression of STAT5A dominant negative with co-expression of GFP

    Depositor

    Andrew Brooks

    Article

    Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

    Inserts

    Stat5a (Stat5a Mouse)
    eGFP
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    C-terminal deletion of 135 nucleotides (dominant …

    Promoter

    CMV

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1α-squirrel-monkey-CHMP3(155)-HA

    Plasmid

    #154173

    Purpose

    expresses squirrel monkey CHMP3(155) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3(155)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 155

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFPC1-Intu

    Plasmid

    #65430

    Purpose

    For mammalian expression of EGFP-mouse Inturned

    Depositor

    Aimin Liu

    Article

    Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

    Insert

    Inturned (Intu Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAMV:nAChRgamma-eGFP

    Plasmid

    #29693

    Depositor

    Henry Lester

    Article

    Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

    Insert

    GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
    

    Use

    Tags

    AMV promotor and poly(A) tail

    Expression

    Bacterial

    Mutation

    eGFP inserted after position S380

    Promoter

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP

    Plasmid

    #154185

    Purpose

    expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA and T2A-H2B-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMXs_FLAG-FSF1

    Plasmid

    #110641

    Purpose

    Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    FSF1 (FSF1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Miro1 S156E

    Plasmid

    #127589

    Purpose

    Phospho-mimetic of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156E

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJL3

    Plasmid

    #184131

    Purpose

    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Pges-1 (3.3kb)

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge