We narrowed to 23,245 results for: Sis

Showing: 9401 - 9440 of 23245 results

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pBSHSP:FLP;CMV:tdTomato-SceI

Plasmid

#30523

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

FLP recombinase tdTomato

Use

Flp/frt, heat shock inducible, i-scei megabase ve…

Mutation

R415H

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pOPTXLUC

Plasmid

#68504

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter (AT1G33440 Mustard Weed)

Tags

luciferase

Expression

Bacterial and Plant

Promoter

OPTX

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pPKm-145

Plasmid

#90505

Purpose

Empty plasmid, pSIN-EF1-alpha-IRES-puro

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Type

Empty backbone

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJP102

Plasmid

#176480

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > NatR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJP103

Plasmid

#176481

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > HygR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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TA0474_pET21a(+)

Plasmid

#13839

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

IS200 transposase (Ta0474 Thermoplasma acidophilum)

Expression

Bacterial

Mutation

See Depositor Comments Below

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRUSH

Plasmid

#54479

Purpose

Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

Depositor

Laurie Jackson-Grusby

Article

Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and RNAi

Tags

SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

Expression

Mammalian

Promoter

U6

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
pT2-cryR;mafbbCE1-P1Egfp

Plasmid

#90155

Purpose

contains mafbb enhancer sequence, and mafbb specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbE1P1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(1-369)

Plasmid

#78753

Purpose

To overexpress MKRN1(1-369) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-369

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-Emi1_control UTRs

Plasmid

#67925

Purpose

Expresses mCherry-Emi1 with control UTRs in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-Emi1 (FBXO5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE658

Plasmid

#153256

Purpose

M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBGT

Plasmid

#62667

Purpose

Donor vector for iRFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iRFP

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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Ad-shMKP-3

Plasmid

#131530

Purpose

Silence the MKP-3 expression

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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FT1

Plasmid

#31435

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Fused Toes Homolog (AKTIP Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pETM6-At4CL-PhCHS-MsCHI

Plasmid

#73392

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dN

Plasmid

#176465

Purpose

express VPS13D lacking N-terminal 117 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT1

Plasmid

#60038

Purpose

Mammalian expression of FLAG-tagged mouse TUT1

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT1 (Mtpap Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
3445 pcDNA3 Bid p15

Plasmid

#8812

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p15 (Bid Mouse)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBDT

Plasmid

#62660

Purpose

Targeting vector for PITT landing pad knock-in into the Rosa26 locus

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

eGFP
neomycin

Use

Mouse Targeting

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1 R mut

Plasmid

#27159

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R box mutant: K249A/V250A/E251A

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
RCAS (A) Hoxd13 (CT#441)

Plasmid

#13985

Depositor

Cliff Tabin

Article

Goff et al Development. 1997 Feb . 124(3):627-36.

Insert

Hoxd-13 (HOXD13 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pIS47 C20orf139 3'UTR

Plasmid

#14489

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-U63-tgRNA-Rev

Plasmid

#112811

Purpose

gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2 V1H (P#663)

Plasmid

#14665

Depositor

Matija Peterlin

Article

Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

Insert

V1H (ATP6V1H Human)

Tags

Gal4 AD

Expression

Yeast

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pMAL-C2X dAmp/Kana+-MT

Plasmid

#154354

Purpose

Bacterial expression of MT

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MT

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X dAmp/Kana+-MTn

Plasmid

#154355

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

His

Expression

Bacterial

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-C2X dAmp/Kana+-MTbeta

Plasmid

#154357

Purpose

Bacterial expression of MTbeta

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTbeta

Tags

His

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,245 results for: Sis